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My email is regarding Abcam primary antibody for SATB2 (rabbit polycolonal) with Cat # ab34735
I have purchased this antibody body (as well as other antibodies from your extensive catalogue) and it worked well showing the desired ˜85kDa band for my C2C12 and primary mouse myoblast protein lysates
However, the new vial I purchased has failed multiple times of probing. I am emailing to ask whether there is a known problem with this Lot# and what do you propose can be done to solve this? It would be a shame if I have to abandon this antibody and search for it in another catalogue.
I’d appreciate a reply as soon as it is possible from you.
Asked on Mar 23 2012
Thank you for contacting us. I am sorry to hear that this batch is not working for your western blot. It is certainly possible that there may be a batch specific issue with the lot you received. If that is the case and you have purchased the antibody in the past six months, I will be happy to send you a free of charge replacement with a new lot, or offer a credit or refund.
It would be very helpful for our complaints investigation if you could please provide some details of your protocol and results. Were you getting high background, no signal, or an incorrect molecular weight? It would be very helpful if you could send an image of your blot. What dilutions and incubation times were used with the primary and secondary antibodies? What was the blocking agent and how were the samples prepared? Was thisa reduced / denatured blot? What was your original order number?
I will be happy to assist you further once I have this additional information.
Answered on Mar 23 2012