Overview

  • Product name
  • Description
    Rabbit polyclonal to SATB2
  • Host species
    Rabbit
  • Specificity
    ab69995 does not cross-react with SATB1.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 16 amino acid peptide near the amino terminus of the human SATB2.

  • Positive control
    • Mouse brain tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab69995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 82 kDa).

Target

  • Function
    Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
  • Tissue specificity
    High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
  • Involvement in disease
    Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
  • Sequence similarities
    Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
  • Cellular localization
    Nucleus matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA binding protein SATB2 antibody
    • DNA-binding protein SATB2 antibody
    • FLJ21474 antibody
    • FLJ32076 antibody
    • GLSS antibody
    • KIAA1034 antibody
    • MGC119474 antibody
    • MGC119477 antibody
    • SATB family member 2 antibody
    • SATB homeobox 2 antibody
    • SATB2 antibody
    • SATB2_HUMAN antibody
    • Special AT rich sequence binding protein 2 antibody
    • Special AT-rich sequence-binding protein 2 antibody
    see all

Images

  • ab69995 at 5 µg/ml staining SATB2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Lane 1 : Anti-SATB2 antibody (ab69995) at 2 µg/ml
    Lane 2 : Anti-SATB2 antibody (ab69995) at 4 µg/ml

    All lanes : Mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 82 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence of SATB2 in Human Brain cells using ab69995 at 20 ug/ml.

References

This product has been referenced in:
  • He L  et al. Expression and localization of special AT-rich sequence binding protein 2 in murine molar development and the pulp-dentin complex of human healthy teeth and teeth with pulpitis. Exp Ther Med 14:3507-3512 (2017). Read more (PubMed: 29042940) »
  • Schütz B  et al. Satb2-Independent Acquisition of the Cholinergic Sudomotor Phenotype in Rodents. Cell Mol Neurobiol N/A:N/A (2014). Rat . Read more (PubMed: 25239161) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

Please could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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