Key features and details
- Rabbit polyclonal to SATB2
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SATB2 antibody
See all SATB2 primary antibodies
DescriptionRabbit polyclonal to SATB2
Specificityab69998 does not cross-react with SATB1.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic 14 amino acid peptide near the carboxy terminus of the human SATB2 (GenBank accession no. Q9UPW6).
- A20 cell lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 82, 65 kDa (predicted molecular weight: 82 kDa).|
FunctionBinds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
Tissue specificityHigh expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
Involvement in diseaseNote=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
Sequence similaritiesBelongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain.
modificationsSumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
Cellular localizationNucleus matrix.
- Information by UniProt
- DNA binding protein SATB2 antibody
- DNA-binding protein SATB2 antibody
- FLJ21474 antibody
ab69998 at 5 µg/ml staining SATB2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
Lane 1 : Anti-SATB2 antibody (ab69998) at 2 µg/ml
Lane 2 : Anti-SATB2 antibody (ab69998) at 4 µg/ml
All lanes : A20 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 82 kDa
Observed band size: 65,82 kDa why is the actual band size different from the predicted?
Immunofluorescence of SATB2 in Human Brain cells using ab69998 at 50 ug/ml.
ab69998 has not yet been referenced specifically in any publications.