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Thanks for your help. I did finally get a signal after letting the exposure go for much longer, so it looks like it worked. I would like to get a 2nd SATB2 antibody to use to confirm the IP I plan to do. Can you recommend another SATB2 antibody that has been successful in Western analysis or IP?
Asked on Nov 08 2011
Thank you for your email. I am very glad to hear that you obtained the desired results. As for another SATB2 antibody that is suitable for IP/WB, we have also ab92446: https://www.abcam.com/SATB2-antibody-EPNCIR130A-ab92446.html It was tested in IP and WB and several other applications, as well as in human, mouse and rat samples. The antibody you are currently using is also suitable for IP: ab51502 Other antibodies tested in WB and human samples are: ab69995, ab69998 and ab119118. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Nov 08 2011