Western blot abreview for Anti-SATB2 antibody [EPNCIR130A]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB231, H1299, SHSY5Y and HEK293T cells)
Loading amount
30 µg
Specification
MDA-MB231, H1299, SHSY5Y and HEK293T cells
Gel Running Conditions
Reduced Denaturing (10 % PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Tris-Saline-Tween with 5% milk

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG HRP-conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
SuperSignal West Pico Chemiluminescent
Exposure
10 second(s)
Bands
Specific: 81 kDa

Additional data

Additional Notes
SHSY5Y cells have highest amount of SATB2 and it can be detected at significant levels in many other cell lines.

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Submitted Jun 02 2012

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