Immunocytochemistry/ Immunofluorescence abreview for Anti-SATB2 antibody [EPNCIR130A]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MDA-MB231 cells)
Specification
MDA-MB231 cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1 % TritonX-100 for 5 minutes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Other product details

Dilution
1/80
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1X PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 594 Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200

Additional data

Additional Notes
DAPI and SATB2 staining is shown in two panels. Most of SATB2 is in nucleus with a small fraction visible in cytoplasm.

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Submitted Jun 02 2012

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