Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SATB2 antibody [EPNCIR130A]

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (E18 mouse brain)
Specification
E18 mouse brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS with 0.1% Triton-X 100 plus 3% goat
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG from Molecular
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 546
Dilution
1/1000

Additional data

Additional Notes
The section was also stained with DNA dye DAPI.

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Submitted Nov 11 2013

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