Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)

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Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
  • Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
  • Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
  • Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPNCIR130B] to SATB2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free
    See all SATB2 primary antibodies

  • Description

    Rabbit monoclonal [EPNCIR130B] to SATB2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human SATB2 aa 700-800 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HT-1080, Saos-2 and fetal brain cell lysates; Human breast cancer tissue.

  • General notes

    ab212176 is the carrier-free version of ab133328 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab212176 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of John Niederhuber. View antibodies from NCI Center for Cancer Research Collaboration.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab212176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Target

  • Function

    Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

  • Tissue specificity

    High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.

  • Involvement in disease

    Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).

  • Sequence similarities

    Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.

  • Post-translational
    modifications

    Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.

  • Cellular localization

    Nucleus matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • DNA binding protein SATB2 antibody
      • DNA-binding protein SATB2 antibody
      • FLJ21474 antibody
      • FLJ32076 antibody
      • GLSS antibody
      • KIAA1034 antibody
      • MGC119474 antibody
      • MGC119477 antibody
      • SATB family member 2 antibody
      • SATB homeobox 2 antibody
      • SATB2 antibody
      • SATB2_HUMAN antibody
      • Special AT rich sequence binding protein 2 antibody
      • Special AT-rich sequence-binding protein 2 antibody
      see all

    Images

    • Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      All lanes : Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : SATB2 knockout HAP1 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 83 kDa
      Observed band size: 83 kDa



      This data was developed using ab133328, the same antibody clone in a different buffer formulation.

      Lanes 1 - 2: Merged signal (red and green). Green - ab133328 observed at 83 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab133328 was shown to specifically react with SATB2 in wild-type HAP1 cells as signal was lost in SATB2 knockout cells. Wild-type and SATB2 knockout samples were subjected to SDS-PAGE. ab133328 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      This data was developed using ab133328, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer tissue sections labeling SATB2 with Purified ab133328 at 1:100 dilution (1.57 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
    • Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 0.08 µg/ml (purified) + Saos-2 (Human osteosarcoma epithelial) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 83 kDa



      This data was developed using ab133328, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      Western blot - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      All lanes : Anti-SATB2 antibody [EPNCIR130B] (ab133328) at 1/1000 dilution (unpurified)

      Lane 1 : HT-1080 cell lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 83 kDa



      This data was developed using ab133328, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      This data was developed using ab133328, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labelling SATB2 with unpurified ab133328 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)
      Anti-SATB2 antibody [EPNCIR130B] - BSA and Azide free (ab212176)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab212176? Please let us know so that we can cite the reference in this datasheet.

    ab212176 has not yet been referenced specifically in any publications.

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