Product nameAnti-SATB2 antibody [SATBA4B10] - C-terminal
See all SATB2 primary antibodies
DescriptionMouse monoclonal [SATBA4B10] to SATB2 - C-terminal
Tested applicationsSuitable for: IP, ICC/IF, IHC-FoFr, WB, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human, Zebrafish
Recombinant fragment corresponding to Human SATB2 (C terminal).
- ICC/IF: HeLa cells. WB: NIH/3T3 whole cell lysate. IP: SATB2 IP in NIH/3T3 whole cell lysate.
This product was changed from ascites to tissue culture supernatant on 9th August 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS
Our Abpromise guarantee covers the use of ab51502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||1/100. Predicted molecular weight: 81 kDa.|
|IHC-Fr||1/50 - 1/400.|
|IHC-P||1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionBinds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
Tissue specificityHigh expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
Involvement in diseaseNote=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
Sequence similaritiesBelongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain.
modificationsSumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
Cellular localizationNucleus matrix.
- Information by UniProt
- DNA binding protein SATB2 antibody
- DNA-binding protein SATB2 antibody
- FLJ21474 antibody
ab51502 staining SATB2 in mouse brain tissue sections by IHC-Fr (Frozen sections).
Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.
ab51502 staining SATB2 in human fetal cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10 mM citrate buffer pH 6.0. Samples were then blocked with 10% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 24 hours at 4°C. A Cy3®-conjugated horse anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counterstained with DAPI (blue).
ab51502 staining SATB2 (red) in a mouse cortex e17.5 cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% donkey serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/10) for 90 minutes at 22°C. An Alexa Fluor®555-conjugated Donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.
Green: CTIP2 (ab18465) staining
Blue: TBR1 (ab31940) staining
ab51502 staining cultured rat cortical neuron cells by ICC/IF.
Cells were fixed in PFA, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C. The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400.
ab51502 at a 1/200 dilution staining SATB2 in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by Immunocytochemistry.
Anti-SATB2 antibody [SATBA4B10] - C-terminal (ab51502) at 1/100 dilution + NIH/3T3 (Mouse embryo fibroblast cell line whole cell lysate at 25 µg
anti Mouse IgG antibody at 1/2500 dilution
Predicted band size: 81 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Same results obtained using HT1080 (Human fibrosarcoma cell line) whole cell lysate at 25 µg.
Immunoprecipitation using ab51502 at 1:100 dilution.
Sample: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate 25 µg.
Secondary used at a 1:2500 dilution.
This product has been referenced in:
- Yoon SJ et al. Reliability of human cortical organoid generation. Nat Methods 16:75-78 (2019). Read more (PubMed: 30573846) »
- Choe Y & Pleasure SJ Meningeal Bmps Regulate Cortical Layer Formation. Brain Plast 4:169-183 (2018). Read more (PubMed: 30598868) »