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Signal Transduction Metabolism Amino Acids
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Anti-SBCAD antibody (ab99951)

  • Datasheet
  • SDS
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Western blot - Anti-SBCAD antibody (ab99951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (ab99951)

Key features and details

  • Rabbit polyclonal to SBCAD
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human SBCAD protein (ab130042)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SBCAD antibody
    See all SBCAD primary antibodies
  • Description

    Rabbit polyclonal to SBCAD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human SBCAD aa 180-402 (internal sequence). (BC013756)

  • Positive control

    • Human fetal liver cell lysate, human fetal colon tissue
  • General notes

    Previously labelled as ACADSB.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized: Add 200ul Steriled Distilled Water.
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SBCAD protein (ab130042)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P
1/100 - 1/500.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P
1/100 - 1/500.

Target

  • Function

    Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent.
  • Tissue specificity

    Ubiquitous.
  • Pathway

    Lipid metabolism; mitochondrial fatty acid beta-oxidation.
  • Involvement in disease

    Defects in ACADSB are the cause of short/branched-chain acyl-CoA dehydrogenase deficiency (SBCADD) [MIM:610006]; also known as 2-methylbutyryl-CoA dehydrogenase deficiency or 2-methylbutyryl glycinuria. SBCADD is an autosomal recessive disorder and consists of a defect in catabolism of L-isoleucine which is characterized by an increase of 2-methylbutyrylglycine and 2-methylbutyrylcarnitine in blood and urine. Affected individuals have seizures and psychomotor delay as the main clinical features.
  • Sequence similarities

    Belongs to the acyl-CoA dehydrogenase family.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P45954 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 36 Human
    • Entrez Gene: 66885 Mouse
    • Entrez Gene: 25618 Rat
    • Omim: 600301 Human
    • SwissProt: P45954 Human
    • SwissProt: Q9DBL1 Mouse
    • SwissProt: P70584 Rat
    • Unigene: 81934 Human
    • Unigene: 334274 Mouse
    • Unigene: 44423 Rat
    see all
  • Alternative names

    • 2 MEBCAD antibody
    • 2 methyl branched chain acyl CoA dehydrogenase antibody
    • 2 methylbutyryl CoA dehydrogenase antibody
    • 2 methylbutyryl coenzyme A dehydrogenase antibody
    • 2-MEBCAD antibody
    • 2-methyl branched chain acyl-CoA dehydrogenase antibody
    • 2-methylbutyryl-CoA dehydrogenase antibody
    • 2-methylbutyryl-coenzyme A dehydrogenase antibody
    • ACAD7 antibody
    • ACADSB antibody
    • ACDSB_HUMAN antibody
    • acyl CoA dehydrogenase, short/branched chain antibody
    • acyl Coenzyme A dehydrogenase short branched chain antibody
    • mitochondrial antibody
    • OTTHUMP00000020685 antibody
    • OTTHUMP00000046795 antibody
    • SBCAD antibody
    • Short/branched chain specific acyl-CoA dehydrogenase antibody
    • short/branched chain specific acyl-CoA dehydrogenase, mitochondrial antibody
    see all

Images

  • Western blot - Anti-SBCAD antibody (ab99951)
    Western blot - Anti-SBCAD antibody (ab99951)
    Anti-SBCAD antibody (ab99951) at 1/500 dilution + Human fetal liver lysate

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (ab99951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (ab99951)

    ab99951, at 1/100 dilution, staining SBCAD in human fetal colon.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab99951? Please let us know so that we can cite the reference in this datasheet.

ab99951 has been referenced in 1 publication.

  • Ericksen RE  et al. Loss of BCAA Catabolism during Carcinogenesis Enhances mTORC1 Activity and Promotes Tumor Development and Progression. Cell Metab 29:1151-1165.e6 (2019). PubMed: 30661928

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