Anti-SBCAD antibody (ab99951)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SBCAD antibody
See all SBCAD primary antibodies -
Description
Rabbit polyclonal to SBCAD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human SBCAD aa 180-402 (internal sequence). (BC013756)
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Positive control
- Human fetal liver cell lysate, human fetal colon tissue
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General notes
Previously labelled as ACADSB.
Properties
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Form
Lyophilised: Add 200ul Steriled Distilled Water. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab99951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 47 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent. -
Tissue specificity
Ubiquitous. -
Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
Involvement in disease
Defects in ACADSB are the cause of short/branched-chain acyl-CoA dehydrogenase deficiency (SBCADD) [MIM:610006]; also known as 2-methylbutyryl-CoA dehydrogenase deficiency or 2-methylbutyryl glycinuria. SBCADD is an autosomal recessive disorder and consists of a defect in catabolism of L-isoleucine which is characterized by an increase of 2-methylbutyrylglycine and 2-methylbutyrylcarnitine in blood and urine. Affected individuals have seizures and psychomotor delay as the main clinical features. -
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 36 Human
- Entrez Gene: 66885 Mouse
- Entrez Gene: 25618 Rat
- Omim: 600301 Human
- SwissProt: P45954 Human
- SwissProt: Q9DBL1 Mouse
- SwissProt: P70584 Rat
- Unigene: 81934 Human
see all -
Alternative names
- 2 MEBCAD antibody
- 2 methyl branched chain acyl CoA dehydrogenase antibody
- 2 methylbutyryl CoA dehydrogenase antibody
see all
Images
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Western blot - Anti-SBCAD antibody (ab99951)Anti-SBCAD antibody (ab99951) at 1/500 dilution + Human fetal liver lysate
Predicted band size: 47 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (ab99951)ab99951, at 1/100 dilution, staining SBCAD in human fetal colon.
References
ab99951 has not yet been referenced specifically in any publications.
