Anti-SBCAD antibody (ab99951)

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Western blot - Anti-SBCAD antibody (ab99951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (ab99951)

Key features and details

  • Rabbit polyclonal to SBCAD
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SBCAD antibody
    See all SBCAD primary antibodies

  • Description

    Rabbit polyclonal to SBCAD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment corresponding to Human SBCAD aa 180-402 (internal sequence). (BC013756)

  • Positive control

    • Human fetal liver cell lysate, human fetal colon tissue

  • General notes

    Previously labelled as ACADSB.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab99951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P
1/100 - 1/500.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P
1/100 - 1/500.

Target

  • Function

    Has greatest activity toward short branched chain acyl-CoA derivative such as (s)-2-methylbutyryl-CoA, isobutyryl-CoA, and 2-methylhexanoyl-CoA as well as toward short straight chain acyl-CoAs such as butyryl-CoA and hexanoyl-CoA. Can use valproyl-CoA as substrate and may play a role in controlling the metabolic flux of valproic acid in the development of toxicity of this agent.

  • Tissue specificity

    Ubiquitous.

  • Pathway

    Lipid metabolism; mitochondrial fatty acid beta-oxidation.

  • Involvement in disease

    Defects in ACADSB are the cause of short/branched-chain acyl-CoA dehydrogenase deficiency (SBCADD) [MIM:610006]; also known as 2-methylbutyryl-CoA dehydrogenase deficiency or 2-methylbutyryl glycinuria. SBCADD is an autosomal recessive disorder and consists of a defect in catabolism of L-isoleucine which is characterized by an increase of 2-methylbutyrylglycine and 2-methylbutyrylcarnitine in blood and urine. Affected individuals have seizures and psychomotor delay as the main clinical features.

  • Sequence similarities

    Belongs to the acyl-CoA dehydrogenase family.

  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 2 MEBCAD antibody
    • 2 methyl branched chain acyl CoA dehydrogenase antibody
    • 2 methylbutyryl CoA dehydrogenase antibody
    • 2 methylbutyryl coenzyme A dehydrogenase antibody
    • 2-MEBCAD antibody
    • 2-methyl branched chain acyl-CoA dehydrogenase antibody
    • 2-methylbutyryl-CoA dehydrogenase antibody
    • 2-methylbutyryl-coenzyme A dehydrogenase antibody
    • ACAD7 antibody
    • ACADSB antibody
    • ACDSB_HUMAN antibody
    • acyl CoA dehydrogenase, short/branched chain antibody
    • acyl Coenzyme A dehydrogenase short branched chain antibody
    • mitochondrial antibody
    • OTTHUMP00000020685 antibody
    • OTTHUMP00000046795 antibody
    • SBCAD antibody
    • Short/branched chain specific acyl-CoA dehydrogenase antibody
    • short/branched chain specific acyl-CoA dehydrogenase, mitochondrial antibody
    see all

Images

  • Western blot - Anti-SBCAD antibody (ab99951)
    Western blot - Anti-SBCAD antibody (ab99951)
    Anti-SBCAD antibody (ab99951) at 1/500 dilution + Human fetal liver lysate

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (ab99951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SBCAD antibody (ab99951)

    ab99951, at 1/100 dilution, staining SBCAD in human fetal colon.

References (1)

Publishing research using ab99951? Please let us know so that we can cite the reference in this datasheet.

ab99951 has been referenced in 1 publication.

  • Ericksen RE  et al. Loss of BCAA Catabolism during Carcinogenesis Enhances mTORC1 Activity and Promotes Tumor Development and Progression. Cell Metab 29:1151-1165.e6 (2019). PubMed: 30661928

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