Overview

  • Product name

  • Description

    Rabbit polyclonal to SBDS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to Human SBDS aa 1-250.
    Database link: Q9Y3A5

  • Positive control

    • Recombinant Human SBDS protein (ab99957) can be used as a positive control in WB. 293T, A431, H1299, HepG2, Molt-4, Raji and Mouse liver lysate, HeLa cells, H1299 xenograft tissue.
  • General notes

    Previously labelled as Shwachman Bodian-Diamond syndrome. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.
  • Tissue specificity

    Widely expressed.
  • Involvement in disease

    Defects in SBDS are the cause of Shwachman-Diamond syndrome (SDS) [MIM:260400]. SDS is an autosomal recessive disorder characterized by pancreatic exocrine insufficiency, hematologic dysfunction, and skeletal abnormalities.
  • Sequence similarities

    Belongs to the SDO1/SBDS family.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Primarily detected in the cytoplasm, and at low levels in nucleus and nucleolus (PubMed:19602484 and PubMed:17475909). Detected in the nucleolus during G1 and G2 phase of the cell cycle, and diffusely distributed in the nucleus during S phase. Detected at the mitotic spindle. Colocalizes with the microtubule organizing center during interphase.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4733401P19Rik antibody
    • AI836084 antibody
    • CGI 97 antibody
    • CGI-97 antibody
    • FLJ10917 antibody
    • MGC105922 antibody
    • Protein 22A3 antibody
    • Ribosome maturation protein SBDS antibody
    • sbds antibody
    • SBDS_HUMAN antibody
    • SDS antibody
    • Shwachman Bodian Diamond syndrome protein antibody
    • Shwachman Bodian Diamond syndrome protein homolog antibody
    • Shwachman Bodian-Diamond syndrome antibody
    • Shwachman-Bodian-Diamond syndrome protein antibody
    • SWDS antibody
    see all

Images

  • All lanes : Anti-SBDS antibody (ab154222) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Anti-SBDS antibody (ab154222) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labelling SBDS with ab154222 at 1/500 dilution (top image), and costained with Hoescht 33342 (bottom image)

  • Immunohistochemical analysis of paraffin embedded Human H1299 xenograft tissue labelling SBDS with ab154222 at a 1/500 dilution.

References

ab154222 has not yet been referenced specifically in any publications.

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