Recombinant
RabMAb

Recombinant Anti-SBDS antibody [EPR7819] (ab128905)

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Overview

  • Product name

    Anti-SBDS antibody [EPR7819]
    See all SBDS primary antibodies

  • Description

    Rabbit monoclonal [EPR7819] to SBDS

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human SBDS aa 1-100. The exact sequence is proprietary.

  • Positive control

    • 293T and Caco 2 cell lysates

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Previously labelled as Shwachman Bodian-Diamond syndrome. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 7.40 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR7819

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab128905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
ICC 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.

    • Target

    • Function

      Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFTUD1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.

    • Tissue specificity

      Widely expressed.

    • Involvement in disease

      Defects in SBDS are the cause of Shwachman-Diamond syndrome (SDS) [MIM:260400]. SDS is an autosomal recessive disorder characterized by pancreatic exocrine insufficiency, hematologic dysfunction, and skeletal abnormalities.

    • Sequence similarities

      Belongs to the SDO1/SBDS family.

    • Cellular localization

      Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm > cytoskeleton > spindle. Primarily detected in the cytoplasm, and at low levels in nucleus and nucleolus (PubMed:19602484 and PubMed:17475909). Detected in the nucleolus during G1 and G2 phase of the cell cycle, and diffusely distributed in the nucleus during S phase. Detected at the mitotic spindle. Colocalizes with the microtubule organizing center during interphase.
    • Information by UniProt

    • Database links

      • Alternative names

        • 4733401P19Rik antibody
        • AI836084 antibody
        • CGI 97 antibody
        • CGI-97 antibody
        • FLJ10917 antibody
        • MGC105922 antibody
        • Protein 22A3 antibody
        • Ribosome maturation protein SBDS antibody
        • sbds antibody
        • SBDS_HUMAN antibody
        • SDS antibody
        • Shwachman Bodian Diamond syndrome protein antibody
        • Shwachman Bodian Diamond syndrome protein homolog antibody
        • Shwachman Bodian-Diamond syndrome antibody
        • Shwachman-Bodian-Diamond syndrome protein antibody
        • SWDS antibody
        see all

      Images

      • Western blot - Anti-SBDS antibody [EPR7819] (ab128905)
        Western blot - Anti-SBDS antibody [EPR7819] (ab128905)
        All lanes : Anti-SBDS antibody [EPR7819] (ab128905) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : CaCo 2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 29 kDa

      • Other - Anti-SBDS antibody [EPR7819] (ab128905)
        Other - Anti-SBDS antibody [EPR7819] (ab128905)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab128905 has not yet been referenced specifically in any publications.

      Publishing research using ab128905? Please let us know so that we can cite the reference in this datasheet.

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