Overview

  • Product name
    Anti-Sbf1 antibody [EPR12566]
    See all Sbf1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12566] to Sbf1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Sbf1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: O95248

  • Positive control
    • Raji, Ramos, Daudi and Human fetal brain lysates; permeabilized Ramos cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR12566
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 208 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Relevance
      Probable pseudophosphatase. Lacks several amino acids in the catalytic pocket which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. May function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • C22:RP4-579N16.2 antibody
      • CMT4B3 antibody
      • DENN/MADD domain containing 7A antibody
      • DENND7A antibody
      • MTMR5 antibody
      • Myotubularin related 5 antibody
      • Myotubularin-related protein 5 antibody
      • SET-binding factor 1 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized Ramos cells labeling Sbf1 with ab177146 at 1/10 dilution (red), or a rabbit IgG (negative) (green).

    • All lanes : Anti-Sbf1 antibody [EPR12566] (ab177146) at 1/1000 dilution

      Lane 1 : Raji lysate
      Lane 2 : Ramos lysate
      Lane 3 : Daudi lysate
      Lane 4 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 208 kDa

    References

    This product has been referenced in:
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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