Overview

  • Product name

    Anti-Sbf1 antibody [EPR12567]
    See all Sbf1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12567] to Sbf1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Sbf1 aa 1750-1850. The exact sequence is proprietary.
    Database link: O95248

  • Positive control

    • WB: Raji, Human brain, Mouse cerebellum, and Mouse testis lysates. IHC-P: Human brain and muscle, and mouse cerebrum tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12567
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 208 kDa (predicted molecular weight: 208 kDa).
IHC-P 1/2000.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

Target

  • Relevance

    Probable pseudophosphatase. Lacks several amino acids in the catalytic pocket which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. May function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • C22:RP4-579N16.2 antibody
    • CMT4B3 antibody
    • DENN/MADD domain containing 7A antibody
    • DENND7A antibody
    • MTMR5 antibody
    • Myotubularin related 5 antibody
    • Myotubularin-related protein 5 antibody
    • SET-binding factor 1 antibody
    see all

Images

  • All lanes : Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/1000 dilution (Purified)

    Lane 1 : Mouse cerebellum lysates
    Lane 2 : Mouse testis lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 208 kDa
    Observed band size: 208 kDa



    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Sbf1 with purified ab181106 at 1/2000 dilution (0.54 µg/ml). Heat mediated antigen retrieval using Bond Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • All lanes : Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/1000 dilution (Purified)

    Lane 1 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
    Lane 2 : Human brain lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 208 kDa
    Observed band size: 208 kDa



    We are unsure how to define the extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Sbf1 with purified ab181106 at 1/2000 dilution (0.54 µg/ml). Heat mediated antigen retrieval using Bond Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Sbf1 with unpurified ab181106 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling Sbf1 with unpurified ab181106 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

References

This product has been referenced in:

See 1 Publication for this product

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