Anti-SBNO1 antibody (ab122789)
Key features and details
- Rabbit polyclonal to SBNO1
- Suitable for: ICC, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SBNO1 antibody -
Description
Rabbit polyclonal to SBNO1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to N terminal amino acids 13-130 of Human SBNO1.
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Positive control
- IHC-P: Human testis, cerebral cortex, lymphoid and endometrium colon tissue; ICC: A549 whole cells; WB: K562 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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ICC
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Sequence similarities
Belongs to the SBNO family. - Information by UniProt
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Database links
- Entrez Gene: 55206 Human
- Omim: 614274 Human
- SwissProt: A3KN83 Human
- Unigene: 7012 Human
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Alternative names
- FLJ10701 antibody
- FLJ10833 antibody
- FLJ16176 antibody
see all
Images
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Immunohistochemical analysis of human testis tissue labeling SBNO1 in the nucleus of cells in seminiferous ducts with ab122789 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemical analysis of A549 (Human lung carcinoma cell line) whole cells labeling SBNO1 in the nucleus of cells in seminiferous ducts with ab122789 at 2 µg/ml.
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Anti-SBNO1 antibody (ab122789) at 0.04 µg/ml + K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Western blot analysis of K562 cell lysate labeling SNBO1 with ab122789 at 0.04 ug/ml.
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Immunohistochemical analysis of human cerebral cortex tissue labeling SBNO1 in the nucleus of neurons with ab122789 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human lymphoid tissue labeling SBNO1 in the nucleus of germinal center cells with ab122789 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human endometrium tissue labeling SBNO1 in the nucleus of glandular cells with ab122789 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab122789 has been referenced in 2 publications.
- Chi F et al. Glycolysis-Independent Glucose Metabolism Distinguishes TE from ICM Fate during Mammalian Embryogenesis. Dev Cell 53:9-26.e4 (2020). PubMed: 32197068
- Watanabe Y et al. Notch and Hippo signaling converge on Strawberry Notch 1 (Sbno1) to synergistically activate Cdx2 during specification of the trophectoderm. Sci Rep 7:46135 (2017). PubMed: 28401892