Anti-SC35 antibody (ab28428)
Key features and details
- Rabbit polyclonal to SC35
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SC35 antibody
See all SC35 primary antibodies -
Description
Rabbit polyclonal to SC35 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant full length protein (Human)
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Positive control
- HeLa nuclear extract
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.9
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Affinity purified IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6427 Human
- Omim: 600813 Human
- SwissProt: Q01130 Human
- Unigene: 584801 Human
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Alternative names
- 35 kDa antibody
- arginine/serine-rich 2 antibody
- PR264 antibody
see all
Images
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All lanes : Anti-SC35 antibody (ab28428)
Lane 1 : recombinant SC35 protein
Lane 2 : HeLa nuclear lysate
Predicted band size: 26 kDa -
IHC image of ab28428 staining in human seminoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28428, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab28428 has been referenced in 8 publications.
- Zhu Z et al. Hsa_circ_0006916 Knockdown Represses the Development of Hepatocellular Carcinoma via Modulating miR-599/SRSF2 Axis. Onco Targets Ther 13:11301-11313 (2020). PubMed: 33177838
- Ilik IA et al. SON and SRRM2 are essential for nuclear speckle formation. Elife 9:N/A (2020). PubMed: 33095160
- Komeno Y et al. SRSF2 Is Essential for Hematopoiesis, and Its Myelodysplastic Syndrome-Related Mutations Dysregulate Alternative Pre-mRNA Splicing. Mol Cell Biol 35:3071-82 (2015). PubMed: 26124281
- Erkelenz S et al. Balanced splicing at the Tat-specific HIV-1 3'ss A3 is critical for HIV-1 replication. Retrovirology 12:29 (2015). PubMed: 25889056
- Rampal R et al. DNA hydroxymethylation profiling reveals that WT1 mutations result in loss of TET2 function in acute myeloid leukemia. Cell Rep 9:1841-55 (2014). PubMed: 25482556
- Berulava T et al. FTO levels affect RNA modification and the transcriptome. Eur J Hum Genet : (2012). PubMed: 22872099
- Kralovicova J et al. Compensatory signals associated with the activation of human GC 5' splice sites. Nucleic Acids Res : (2011). PubMed: 21609956
- Toyota M et al. Epigenetic silencing of microRNA-34b/c and B-cell translocation gene 4 is associated with CpG island methylation in colorectal cancer. Cancer Res 68:4123-32 (2008). WB ; Human . PubMed: 18519671