Key features and details
- Rabbit polyclonal to SC35 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SC35 antibody - N-terminal
See all SC35 primary antibodies
DescriptionRabbit polyclonal to SC35 - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Chimpanzee, Rhesus monkey
Synthetic peptide within Human SC35 (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q01130
- WB: HL-60 whole cell extract. IHC-P: Mouse lung tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionNecessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
Sequence similaritiesBelongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain.
modificationsExtensively phosphorylated on serine residues in the RS domain.
- Information by UniProt
- 35 kDa antibody
- arginine/serine-rich 2 antibody
- PR264 antibody
Anti-SC35 antibody - N-terminal (ab229473) at 1/1000 dilution + HL-60 (human romyelocytic leukemia cell line) whole cell extract at 30 µg
Predicted band size: 25 kDa
12% SDS-PAGE gel.
Paraffin-embedded mouse lung tissue stained for SC35 using ab229473 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229473 has not yet been referenced specifically in any publications.