Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)
Overview
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Product name
Anti-SC35 antibody [SC-35] - BSA and Azide free
See all SC35 primary antibodies -
Description
Mouse monoclonal [SC-35] to SC35 - BSA and Azide free -
Host species
Mouse -
Specificity
In immunohistology and immunoelectronmicroscopy, the antibody labels SC-35 as a speckled pattern that occupies a portion of the nucleoplasm, excluding the nucleoli. Nie et al (2002) report that "In human endometrium the SC35 protein was detected by western blot analysis and had a molecular mass of approximately 90 kDa, indicating that in the endometrial extracts, the 35 kDa protein remains bound to one or more components of the spliceosome."
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Tested applications
Suitable for: ELISA, IHC-P, ICC, ICC/IFmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Rat, Human, Xenopus laevis, Drosophila melanogaster, Rhesus monkey, Newt -
Epitope
This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. -
Positive control
- ICC/IF: HeLa cells.
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General notes
ab264431 is the PBS only version of ab11826.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
SC-35 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab264431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | 1/500. | |
| ICC | 1/2000. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| AP | Use at an assay dependent concentration. |
Target
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Function
Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6427 Human
- Entrez Gene: 494445 Rat
- Entrez Gene: 708105 Rhesus monkey
- Omim: 600813 Human
- SwissProt: Q01130 Human
- SwissProt: Q6PDU1 Rat
- Unigene: 584801 Human
- Unigene: 136644 Rat
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Alternative names
- 35 kDa antibody
- arginine/serine-rich 2 antibody
- PR264 antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)](https://www.abcam.com/ps/products/264/ab264431/Images/ab264431-355621-AntiSC35-antibody-SC35--BSA-and-Azide-free-immunocytochemistry-hela-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)This image was produced using the same antibody clone but in a different formulation ab11826.
ICC/IF image of ab11826 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11826 at 1 µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References
ab264431 has not yet been referenced specifically in any publications.
