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    sc35-antibody-sc-35-nuclear-speckle-marker-ab11826.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

  • Datasheet
  • SDS
Reviews (15)Q&A (16)References (88)

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Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

Key features and details

  • Mouse monoclonal [SC-35] to SC35 - Nuclear Speckle Marker
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

You may also be interested in

Primary
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Anti-Coilin antibody [Pdelta] (ab11822)
Protein
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Recombinant Human SC35 protein (ab152674)
Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker
    See all SC35 primary antibodies
  • Description

    Mouse monoclonal [SC-35] to SC35 - Nuclear Speckle Marker
  • Host species

    Mouse
  • Specificity

    This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF.  Recent data suggests this clone may cross-react with additional proteins within the spliceosome complex (PMID: 33095160)

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Other Immunogen Type. Fractioned spliceosome complex (PMID: 2137203)

  • Positive control

    • ICC/IF: MCF7, NIH3T3 and Rin-5F cells
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    SC-35
  • Isotype

    IgG1
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Other Nuclear Bodies

Associated products

  • Alternative Versions

    • Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human SC35 protein (ab152674)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Mouse
Rat
Human
All applications
Xenopus laevis
Drosophila melanogaster
Rhesus monkey
Newt
Application Abreviews Notes
ICC/IF (8)
Use a concentration of 5 µg/ml.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
    • Sequence similarities

      Belongs to the splicing factor SR family.
      Contains 1 RRM (RNA recognition motif) domain.
    • Post-translational
      modifications

      Extensively phosphorylated on serine residues in the RS domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q01130 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6427 Human
      • Entrez Gene: 20382 Mouse
      • Entrez Gene: 494445 Rat
      • Entrez Gene: 708105 Rhesus monkey
      • Omim: 600813 Human
      • SwissProt: Q01130 Human
      • SwissProt: Q62093 Mouse
      • SwissProt: Q6PDU1 Rat
      • Unigene: 584801 Human
      • Unigene: 21841 Mouse
      • Unigene: 429312 Mouse
      • Unigene: 136644 Rat
      see all
    • Alternative names

      • 35 kDa antibody
      • arginine/serine-rich 2 antibody
      • PR264 antibody
      • Protein PR264 antibody
      • SC 35 antibody
      • SC-35 antibody
      • SC35 antibody
      • Serine/arginine-rich splicing factor 2 antibody
      • SFRS 2 antibody
      • SFRS2 antibody
      • SFRS2A antibody
      • Splicing component 35 kDa antibody
      • Splicing component antibody
      • Splicing factor antibody
      • Splicing factor arginine/serine rich 2 antibody
      • Splicing factor SC35 antibody
      • Splicing speckle antibody
      • Splicing speckles antibody
      • SR splicing factor 2 antibody
      • SRp30b antibody
      • SRSF2 antibody
      • SRSF2_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Lab

      ab11826 staining SC35 - Nuclear Speckle Marker in Rin-5F cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 4% paraformaldehyde (10 min).
    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Lab

      ab11826 staining SC35 - Nuclear Speckle Marker in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Lab

      ab11826 staining SC35 - Nuclear Speckled Marker in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Image from Witek, Matthew E. et al. PLoS ONE 9.8 (2014): e104293. doi: 10.1371/journal.pone.0104293. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunocytochemistry/ Immunofluorescence analysis of HEK-293T and RKO cells transiently transfected with CDX2/AS-His and co-stained for CDX2/AS-His and SC35 (ab11826). All proteins localized to the nucleus and merged images revealed co-localization of CDX2/AS with SC35.

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an abreview submitted by Dr Sam Nowitzki, Barrow Neurological Institute.

      Immunocytochemistry/ Immunofluorescence analysis of HEK-293 human kidney cells labeling SC35 with ab11826 at 1/400 dilution. Cells were fixed with methanol and blocked with PBS for 1 hour at 4°C. Staining with ab11826 was carried out in PBS buffer for 2 hours at 4°C. An undiluted goat anti-mouse Alexa Fluor® 594 secondary antibody was used. 

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Image from Salsman, Jayme et al. PLoS Pathogens 4.7 (2008): e1000100. doi: 10.1371/journal.ppat.1000100. Fig S4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Immunocytochemistry/ Immunofluorescence analysis of untransfected U-2 OS cells (A) and cells transfected with HSV US1 or US1.5 fixed and stained for FLAG (red) and SC35 (green) to identify viral proteins and nuclear speckles respectively. Transfected cells were fixed 40 h post transfection with 3.7% formaldehyde in PBS (20 min), permeabilized with 0.5% Triton X-100 in PBS (10 min), and blocked with 4% BSA in PBS (20 min) prior to incubation with Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826) and secondary antibodies in 4% BSA in PBS. DAPI was used for visualization of nuclear DNA. Scale bar  =  10 µm.

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an Abreview submitted by Dr Eva Bartova

      Immunocytochemistry/ Immunofluorescence analysis of human adenocarcinoma HT-29 (Human colorectal adenocarcinoma cell line) cells labeling SC35 with ab11826 at 1/200 dilution. Cells were fixed in paraformaldehyde and permeabilized with Triton X-100 and Saponin. Blocking of the cells was done with 1% BSA for 1 hour at 37°C; staining with ab11826 at 1/200 was carried out for 16 hours at 4°C in PBS buffer. An anti-mouse IgG3 (Alexa Fluor® 594) secondary antibody was used at 1/200 dilution. 

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an abreview submitted by Dr Sam Nowitzki, Barrow Neurological Institute.

      Immunocytochemistry/ Immunofluorescence analysis of  human hippocampus cells labeling SC35 with ab11826 at 1/200 dilution. Cells were fixed with formaldehyde and blocked with PBS for 1 hour at 4°C. Staining with ab11826 was carried out in PBS buffer for 12 hours at 4°C. A goat anti-mouse Alexa Fluor® 594 secondary antibody was used at 1/1000 dilution. 

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an anonymous Abreview.

      ab11826 staining SC35 in human fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% Normal Goat Serum/0.3% Triton X-100 in PBS for 60 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 1% BSA/ 0.3% Triton X-100 in PBS) for 16 hours at 4°C. An Alexa Flour® 488 goat anti-mouse IgG (H+L), F(ab')2 Fragment Ig was used as the secondary antibody at a dilution of 1/1000.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an Abreview submitted by Dr Kirk McManus

      ab11826 (1/1000) staining SC35 (phospho) in human retinal pigment epithelial (RPE) cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an Abreview submitted by Dr Eva Bartova

      ab11826 staining cultured human colon adenocarcinoma HT-29 cells.

      Cells were PFA fixed and permeabilized in Triton X-100 and saponin prior to blocking with 1% BSA for 1 hour at RT. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 594 conjugated goat anti-mouse IgG3 antibody was used as the secondary.

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Image from de Chiara C et al, PLoS One. 2009 Dec 23;4(12):e8372, Fig 3.

      HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed 24–48 hours after transfection using 4% paraformaldehyde, permeabilized with 0.2% triton X-100/PBS and probed with ab11826 followed by FITC conjugated secondary antibodies (green). After washing with PBS, slides were mounted using Citifluor and analysed by confocal microscopy. Cells were visualized under a Leica laser scanning confocal microscope equipped with a DM-RXE microscope and an argon-krypton laser.

    • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
      Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

      ICC/IF image of ab11826 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab11826 at 1 µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    Protocols

    • Recommended ICC protocol with ab11826
    • Mouse on Mouse staining protocol

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (88)

    Publishing research using ab11826? Please let us know so that we can cite the reference in this datasheet.

    ab11826 has been referenced in 88 publications.

    • Wang Z  et al. Modulation of SRSF2 expression reverses the exhaustion of TILs via the epigenetic regulation of immune checkpoint molecules. Cell Mol Life Sci 77:3441-3452 (2020). PubMed: 31838573
    • Francis AC  et al. HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun 11:3505 (2020). PubMed: 32665593
    • Chen ZH  et al. Nuclear export of chimeric mRNAs depends on an lncRNA-triggered autoregulatory loop in blood malignancies. Cell Death Dis 11:566 (2020). PubMed: 32703936
    • Martynova E  et al. Transcriptomic analysis and novel insights into lens fibre cell differentiation regulated by Gata3. Open Biol 9:190220 (2019). PubMed: 31847788
    • Salas-Armenteros I  et al. Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability. Cell Rep 28:1551-1563.e7 (2019). PubMed: 31390568
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 31 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Primary neuron)
    Permeabilization
    Yes - 0.3%Triton-X100
    Specification
    Primary neuron
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Fuzi Jin

    Verified customer

    Submitted Dec 01 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 25 2019

    Western blot abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Below Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 04 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human fibroblast)
    Permeabilization
    Yes - 0.1%Tx100
    Specification
    human fibroblast
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 24 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.1% TritonX100 in PBS
    Specification
    HEK293
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 27 2018

    Western blot abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Average
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF-7 Whole Cell RIPA Lysate)
    Gel Running Conditions
    Reduced Denaturing (15% BIS-TRIS Gel)
    Loading amount
    40 µg
    Specification
    MCF-7 Whole Cell RIPA Lysate
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 02 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Hippocampus-Paraffin embedded)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Permeabilization
    No
    Specification
    Hippocampus-Paraffin embedded
    Blocking step
    Scy-tek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 16 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (fibroblast)
    Permeabilization
    Yes - block contains 0.3% tritonX-100 (1H, RT)
    Specification
    fibroblast
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 29 2016

    Western blot abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Excellent
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293 (kidney cell))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris Gradient)
    Loading amount
    5 µg
    Specification
    HEK293 (kidney cell)
    Blocking step
    Licor Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 18 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Scytek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Sample
    Human Cell (HEK293 (kidney))
    Specification
    HEK293 (kidney)
    Permeabilization
    No
    Fixative
    Methanol
    Read More

    Dr. Sam Nowitzki

    Verified customer

    Submitted Apr 06 2015

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