Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

Reviews (15)Q&A (16)References (109)
Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

Key features and details

  • Mouse monoclonal [SC-35] to SC35 - Nuclear Speckle Marker
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker
    See all SC35 primary antibodies

  • Description

    Mouse monoclonal [SC-35] to SC35 - Nuclear Speckle Marker

  • Host species

    Mouse

  • Specificity

    This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF.  Recent data suggests this clone may cross-react with additional proteins within the spliceosome complex (PMID: 33095160)

  • Tested applications

    Suitable for: ICC/IFmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis, Drosophila melanogaster, Rhesus monkey, Newt

  • Immunogen

    Other Immunogen Type. Fractioned spliceosome complex (PMID: 2137203)

  • Positive control

    • ICC/IF: MCF7, NIH3T3 and Rin-5F cells

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11826 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (8)
Use a concentration of 5 µg/ml.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
Application notes
Is unsuitable for WB.

Target

  • Function

    Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.

  • Sequence similarities

    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.

  • Post-translational
    modifications

    Extensively phosphorylated on serine residues in the RS domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 35 kDa antibody
    • arginine/serine-rich 2 antibody
    • PR264 antibody
    • Protein PR264 antibody
    • SC 35 antibody
    • SC-35 antibody
    • SC35 antibody
    • Serine/arginine-rich splicing factor 2 antibody
    • SFRS 2 antibody
    • SFRS2 antibody
    • SFRS2A antibody
    • Splicing component 35 kDa antibody
    • Splicing component antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 2 antibody
    • Splicing factor SC35 antibody
    • Splicing speckle antibody
    • Splicing speckles antibody
    • SR splicing factor 2 antibody
    • SRp30b antibody
    • SRSF2 antibody
    • SRSF2_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

    ab11826 staining SC35 - Nuclear Speckle Marker in Rin-5F cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

    ab11826 staining SC35 - Nuclear Speckle Marker in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)

    ab11826 staining SC35 - Nuclear Speckled Marker in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Image from Witek, Matthew E. et al. PLoS ONE 9.8 (2014): e104293. doi: 10.1371/journal.pone.0104293. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence analysis of HEK-293T and RKO cells transiently transfected with CDX2/AS-His and co-stained for CDX2/AS-His and SC35 (ab11826). All proteins localized to the nucleus and merged images revealed co-localization of CDX2/AS with SC35.

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an abreview submitted by Dr Sam Nowitzki, Barrow Neurological Institute.

    Immunocytochemistry/ Immunofluorescence analysis of HEK-293 human kidney cells labeling SC35 with ab11826 at 1/400 dilution. Cells were fixed with methanol and blocked with PBS for 1 hour at 4°C. Staining with ab11826 was carried out in PBS buffer for 2 hours at 4°C. An undiluted goat anti-mouse Alexa Fluor® 594 secondary antibody was used. 

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Image from Salsman, Jayme et al. PLoS Pathogens 4.7 (2008): e1000100. doi: 10.1371/journal.ppat.1000100. Fig S4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunocytochemistry/ Immunofluorescence analysis of untransfected U-2 OS cells (A) and cells transfected with HSV US1 or US1.5 fixed and stained for FLAG (red) and SC35 (green) to identify viral proteins and nuclear speckles respectively. Transfected cells were fixed 40 h post transfection with 3.7% formaldehyde in PBS (20 min), permeabilized with 0.5% Triton X-100 in PBS (10 min), and blocked with 4% BSA in PBS (20 min) prior to incubation with Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826) and secondary antibodies in 4% BSA in PBS. DAPI was used for visualization of nuclear DNA. Scale bar  =  10 µm.

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an Abreview submitted by Dr Eva Bartova

    Immunocytochemistry/ Immunofluorescence analysis of human adenocarcinoma HT-29 (Human colorectal adenocarcinoma cell line) cells labeling SC35 with ab11826 at 1/200 dilution. Cells were fixed in paraformaldehyde and permeabilized with Triton X-100 and Saponin. Blocking of the cells was done with 1% BSA for 1 hour at 37°C; staining with ab11826 at 1/200 was carried out for 16 hours at 4°C in PBS buffer. An anti-mouse IgG3 (Alexa Fluor® 594) secondary antibody was used at 1/200 dilution. 

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an abreview submitted by Dr Sam Nowitzki, Barrow Neurological Institute.

    Immunocytochemistry/ Immunofluorescence analysis of  human hippocampus cells labeling SC35 with ab11826 at 1/200 dilution. Cells were fixed with formaldehyde and blocked with PBS for 1 hour at 4°C. Staining with ab11826 was carried out in PBS buffer for 12 hours at 4°C. A goat anti-mouse Alexa Fluor® 594 secondary antibody was used at 1/1000 dilution. 

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an anonymous Abreview.

    ab11826 staining SC35 in human fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 in PBS and blocked with 5% Normal Goat Serum/0.3% Triton X-100 in PBS for 60 minutes at 25°C. Samples were incubated with primary antibody (1/500 in 1% BSA/ 0.3% Triton X-100 in PBS) for 16 hours at 4°C. An Alexa Flour® 488 goat anti-mouse IgG (H+L), F(ab')2 Fragment Ig was used as the secondary antibody at a dilution of 1/1000.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an Abreview submitted by Dr Kirk McManus

    ab11826 (1/1000) staining SC35 (phospho) in human retinal pigment epithelial (RPE) cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to abreview for further experimental details.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)This image is courtesy of an Abreview submitted by Dr Eva Bartova

    ab11826 staining cultured human colon adenocarcinoma HT-29 cells.

    Cells were PFA fixed and permeabilized in Triton X-100 and saponin prior to blocking with 1% BSA for 1 hour at RT. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 594 conjugated goat anti-mouse IgG3 antibody was used as the secondary.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)
    Immunocytochemistry/ Immunofluorescence - Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker (ab11826)Image from de Chiara C et al, PLoS One. 2009 Dec 23;4(12):e8372, Fig 3.

    HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed 24–48 hours after transfection using 4% paraformaldehyde, permeabilized with 0.2% triton X-100/PBS and probed with ab11826 followed by FITC conjugated secondary antibodies (green). After washing with PBS, slides were mounted using Citifluor and analysed by confocal microscopy. Cells were visualized under a Leica laser scanning confocal microscope equipped with a DM-RXE microscope and an argon-krypton laser.

References (109)

Publishing research using ab11826? Please let us know so that we can cite the reference in this datasheet.

ab11826 has been referenced in 109 publications.

  • Zepecki JP  et al. miRNA-mediated loss of m6A increases nascent translation in glioblastoma. PLoS Genet 17:e1009086 (2021). PubMed: 33684100
  • Marenda M  et al. Parameter-free molecular super-structures quantification in single-molecule localization microscopy. J Cell Biol 220:N/A (2021). PubMed: 33734291
  • Legartová S  et al. The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction. Cells 10:N/A (2021). PubMed: 33535591
  • Honda M  et al. High-depth spatial transcriptome analysis by photo-isolation chemistry. Nat Commun 12:4416 (2021). PubMed: 34285220
  • Liu X  et al. Time-dependent effect of 1,6-hexanediol on biomolecular condensates and 3D chromatin organization. Genome Biol 22:230 (2021). PubMed: 34404453
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

11-20 of 31 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SC35 antibody [SC-35] - Nuclear Speckle Marker

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Scytek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (Hippocampus (Brain))
Specification
Hippocampus (Brain)
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Sam Nowitzki

Verified customer

Submitted Mar 31 2015

Question

FISH protocol. Antigen retrieval used to validate ab11826 in IHC-P

Read More
Answer

After contacting the laboratory, the IHC-P protocol in the paper through the link below was used successfully with this antibody.

http://www.reproduction-online.org/content/124/2/209.full.pdf

Our recommended ISH protocol is given through the link below:
https://www.abcam.com/index.html?pageconfig=resource&rid=11743

Read More

Question

The end user would like the no charge replacement for this.

I appreciate your patience.

Read More
Answer

Thank you for contacting us again.

we will gladly provide a free of charge replacement, however, we need to know which antibody we shall send to you.

The unwanted cross-reactivity is present in all lots, therefore, we cannot provide a new lot of this antibody as the end -user would come across the same problems as before. Moreover, we do not have an alternative product in our catalog which detects the phophorylated form.

Hence, the replacement antibody will need to be a different product.

Please let us know which one your end user prefers.

Read More

Question

Thank you for your feedback as it is very valuable to us! Our group need to
study the phosphorylation of both SC-35 and also SF2/ASF, so the
cross-reactivity of this antibody would really affect us.
We are now considering the compensation. May I ask if there's new clone/vial of
phospho-SC35 antibody that without the mentioned cross-reactivity? If yes, we
would like to have a replacement. If not, we don't need a refund or credit note
as we used this antibody frequently. Thank you!

Read More
Answer

Thank you for your nice reply.

Unfortunately, it is not only a batch problem, but will happen all the time with this antibody. That's why we removed the application and references for WB. this means unfortunately, that I cannot send you a replacement of this antibody. Moreover, we do not have any antibodies in our catalog which can detect the phosphorylated form.

Thank you very much for your honesty.

I wish you all the best with your future experiments!

Read More

Question

Thank you for your information! We used this antibody mainly for
immunocytochemistry which performed really great. I've also tried it for
Western Blot, the band is sharp and specific but the result is quite unexpected
when we did some quantification. FYI we used HeLa and HEK293 as our samples.
There's no need for the compensation. But may I ask what's the problem for using
it in WB?

Read More
Answer

This antibody works fine for ICC, but we found that in WB it shows a possible cross-reactivity with the SC35-related non-snRNP factor SF2/ASF (p75) and Ad2 proteins. This may explain your unexpected results.

Read More

Question

Buenas tardes
Muchas gracias por tu rápida contestación.
Siguiendo tus instrucciones cambié la leche por BSA al 5% en TBS/Tween0.1% para incubar los anticuerpos (el resto del protocolo lo seguí igual que la vez anterior). Te adjunto el escaner de la foto.
En este caso lo que tengo son un montón de bandas inespecíficas aunque la banda que conseguí la primera vez corresponde a la más intensa (marcada con una flecha).
Me comentaste que la porteína podría estar formando un dímero, pero no tengo constancia de que eso sea posible.
Cómo puedo enviar tanta inespecificidad?
Muchas gracias por tu ayuda.

Read More
Answer

Perdona por la espera.

La mejor manera de evitar bandas inespecíficas es asegurar unas condiciones de preparación de la muestra idóneas para el anticuerpo en cuestión. Este anticuerpo reconoce el epítopo en condiciones reductoras y desnaturalizantes.

Si queréis darle otra oportunidad al anticuerpo y testarlo tal y como se indica en la datasheet, éstas serían las condiciones:

-Buffer de lisis: 6.0% (w/v) SDS, 0.14 mol Tris (pH 6.8), 22.4% (v/v) glycerol, protease inhibitor cocktail.

-Centrifugar a 14000g durante 15minutos a 48C.

-Volver a bloquear con leche esta vez, usando leche desnatada en polvo al 5% en TBST con 0.1% Tween 20 durante 1 hora.

-Incubar el anticuerpo diluido en 5% de leche en TBS con 0.1% de Tween durante 1 hora a TA.

Si preferís directamente recibir un reemplazo gratuito del anticuerpo, siendo que éste está bajo garantía, o un reembolso del mismo, también es posible. Existen lotes en stock diferentes del que habéis recibido y no os ha dado los resultados esperados.

Simplemente indícame como queréis proceder y estaré encantada de ayudaros.

Recuerda que si queréis recibir un nuevo anticuerpo como reemplazo en el mismo pedido podríamos enviaros de forma gratuita otro anticuerpo primario Rabbit Monoclonal, tal y como se especifica en nuestra promoción.

Para más información sobre la misma, visita el siguiente enlace:

https://www.abcam.com/index.html?pageconfig=resource&rid=15447

No dudes en consultarme cualquier otra duda que podáis tener. Gracias por tu colaboración.

Read More

Question

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A. Yes
Q. How did you feel about being given protocol advice?
A. I requested protocol advice
Q. Did the product work successfully after following the advice?
A. No
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A. Yes

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A. No
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A. Very satisfied
Q. Could we have done anything to better resolve the problem?
A. you should show more western blot images for SC-35 in different cell lines. Your antibody gave me a lot of aspecific bands in Mouse cortical Neurons cell lysates
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A. No, I would not mind
Q. Please provide a phone number or email address where we can reach you.
A. mpacif@lsuhsc.edu
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A. Your assistance was really fast and efficient

Read More
Answer

Thank you for participating in our recent customer satisfaction survey. Your complete satisfaction with our products and services is very important to us.

I am happy to hear that you were satisfied with the credit note/refund raised in this regard. We are always aiming to add more testing data to our datasheets, and for ab11826 we only have human testing data from customers so far. https://www.abcam.com/SC35-antibody-SC-35-Nuclear-Speckle-Marker-ab11826.html&tab=abreviews&intabreviewid=13829

But if there is anything else that we could do to help, please let us know.

Have a great weekend!

Read More

Question

Anbei WB-Bilder und der ausgefüllte Fragebogen.

Bei weiteren Fragen stehe ich Ihnen gerne zur Verfügung.

Read More
Answer

Vielen Dank für Ihre Anfrage und Ihre Geduld. Bitte entschuldigen Sie die lange Verzögerung - die Antwort des Labors hat etwas auf sich warten lassen.
Ich weiß, dass Sie schon viel Zeit in Ihre Experimente investiert haben und es ist enttäuschend, dass Sie bisher noch kein positives Ergebnis haben. Um Ihr Problem genauer zu verstehen und lösen zu können, möchte ich Sie noch nach den folgenden Details fragen bzw. die folgenden Vorschläge zu Veränderung Ihres Protokolls machen:
1) Welche Zellen haben Sie untersucht? Benötigt diese Zelllinie eine Induktion der SC35-Expression?
2) Hatten Sie Phosphataseinhibitoren eingesetzt? Dieser Antikörper wird routinemäßig gegen das phosphorylierte Epitop getestet, weshalb leider nicht umfassend charakterisiert ist, ob bzw. wie gut er das nicht-phosphorylierte Epitop erkennt; eine frühe Studie zu diesem Antikörper finden Sie bei Fu et al. (PMID:2137203). Wir würden Ihnen empfehlen, Phosphataseinhibitoren wie Natriumfluorid oder Natriumorthovanadat einzusetzen, um eine optimale Erkennung des Zielproteins zu ermöglichen.
3) Ist der sekundäre Antikörper mit HRP konjugiert, und wie oft oder stark waschen Sie den Blot vor Inkubation mit dem sekundären? Es wurde gezeigt, dass Natriumazid die Peroxidase inhibiert, und wir haben die Erfahrung gemacht, dass selbst kleinste Mengen stören können. Es könnte daher hilfreich sein, auf Natriumazid in den Puffern zu verzichten oder die Anzahl der Waschschritte zu erhöhen.
4) Hatten Sie auch höhere Konzentrationen des Primärantikörpers ausprobiert? Ein anderer Kunde hatte gute Ergebnisse bei einer Verdünnung von 1:300 erzielt (siehe Abreview).
Ich möchte Sie gerne auch noch darauf aufmerksam machen, dass der Antikörper neben SC-35 auch den SC-35-verwandten non-snRNP Faktor SF2/ASF erkennt. Ich hoffe, das ist für Ihre Versuchsauswertung akzeptabel?
Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Sollten oben genannte Vorschläge nicht zum Erfolg führen, werde ich Ihnen natürlich gerne einen Ersatz oder eine Gutschrift schicken. Bitte lassen Sie mich wissen, ob ich Ihnen helfen konnte und zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

Read More

Question

1) Testsample fuer ab11826? Wir moechten ihn gerne fuer unser Protokoll testen. 2) MAP2+Cy5 (chicken-combi) funktioniert bei uns leider nicht (nicht die Gehirnschnitte, sondern Neuronenkultur). Hatten schon sehr niedrige Verduennungen probiert, andere AK funktionieren, aber diese Kombination liefert keinerlei Signale.

Read More
Answer

Vielen Dank für Ihren Anruf. Es tut mir leid zu hören, dass Sie Probleme mit unserem MAP2-Antikörper ab92434 haben. Wie besprochen habe ich unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll sowie für unsere Qualitätskontrolle. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken. Auch wenn ab92434 bislang nur für die Immuncytochemie/Immunfluoreszenz (ICC/IF) garantiert ist, können wir eventuell eine Ausnahme machen, wenn uns genauere Informationen zu Ihrem besonderen Protokoll vorliegen. Details zu unserer Garantie finden Sie unter: https://www.abcam.com/abpromise Wie besprochen sende ich Ihnen auch die Informationen zu unserem Testprogramm: Unseres Wissens nach wurde der SC35-Antikörper ab11826 bisher noch nicht in der Immunhistochemie an Gefrierschnitten (IHC-Fr) getestet (was wie besprochen Ihrem Protokoll vielleicht am nächsten kommt). Falls Sie dies selbst testen möchten, kann ich Ihnen ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden. Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten: 1.) Bestätigen Sie mir bitte, dass Sie ab11826 in der IHC-Fr testen möchten. 2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes. 3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax 4.) Testen Sie den Antikörper in der IHC-Fr. 5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes" Unter der folgenden URL können Sie mehr über unser Abreview System erfahren: https://www.abcam.com/abreviews 6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörper bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss. Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Read More

Question

Is it possible to know the epitope of this mAb for SC-35? Many thanks.

Read More
Answer

Thank you for your enquiry. Unfortunately the epitope of this product has not been mapped. Therefore, we do not have any information on the exact epitope sequence region. I am sorry I can't be of more help in this occasion but if there is anything else that I can help you with, please do not hesitate to contact me.

Read More

11-20 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com