Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Scytek Superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Sample
Human Cell (HEK293 (kidney))
Specification
HEK293 (kidney)
Permeabilization
No
Fixative
Methanol
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Scytek Superblock (100%)
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: Goat-anti-Mouse Alexafluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
This antibody gives a similar staining pattern with methanol or paraformaldehyde fixation. It works very well as a nuclear speckle marker, with high signal only in the nucleus and very low background. Highly recommended for the study of nuclear speckles.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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Verified customer
Submitted Apr 06 2015