Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RPE-1)
Specification
RPE-1
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100 in PBS
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous RPE-1 cells were paraformaldehyde fixed (4%, 10min), permeablized and immunofluorescently labeled. Labeled interphase cells gave a strong nuclear speckled pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
Submitted Aug 15 2007