Key features and details
- Rabbit polyclonal to SC5DL
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SC5DL antibody
DescriptionRabbit polyclonal to SC5DL
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
- ICC/IF: HaCaT cells. WB: Human tonsil and liver lysates; RT4 cell lysates.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab221764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 35 kDa.|
RelevanceSC5DL catalyzes the conversion of lathosterol into 7 dehydrocholesterol. Mutations in this gene have been associated with lathosterolosis.
Cellular localizationEndoplasmic reticulum membrane; Multipass membrane protein
- 3beta hydroxysteroid delta5 desaturase antibody
- C 5 sterol desaturase antibody
- Delta 5 desaturase like antibody
All lanes : Anti-SC5DL antibody (ab221764) at 1/250 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 35 kDa
PFA-fixed, Triton X-100 permeabilized HaCaT (human keratinocyte cell line) cells stained for SC5DL (green) using ab221764 at 4 µg/ml in ICC/IF.
ab221764 has been referenced in 1 publication.
- Koczok K et al. Subcellular localization of sterol biosynthesis enzymes. J Mol Histol 50:63-73 (2019). PubMed: 30535733