Recombinant
RabMAb

Recombinant Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Overview

  • Product name

    Anti-Sca1 / Ly6A/E antibody [EPR3355]
    See all Sca1 / Ly6A/E primary antibodies
  • Description

    Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse Sca1/ Ly6A/E aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: Mouse bone marrow, and Hepa1-6 lysates. IHC-P: mouse kidney tissue.
  • General notes

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended. See IHC antigen retrieval protocols.

For unpurified use at 1/50 - 1/100

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      T-cell activation.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Contains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications

      O-glycosylated. Not N-glycosylated.
      Not phosphorylated.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    Images

    • Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/10000 dilution (Purified) + Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?

    • Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/1000 dilution (Purified) + Mouse bone marrow lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 14 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?

    • ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Natale BV  et al. Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta. Sci Rep 7:5575 (2017). Read more (PubMed: 28717241) »
    • Passaro D  et al. Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia. Cancer Cell 32:324-341.e6 (2017). Read more (PubMed: 28870739) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Uterus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate
    Permeabilization
    Yes - Tween-20
    Specification
    Uterus
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde

    Reshef Tal

    Verified customer

    Submitted Sep 04 2019

    Question
    Answer

    Thank you for contacting us. I have heard back from the lab: For WB, denaturing and reducing conditions were used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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