Recombinant Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)](https://www.abcam.com/ps/products/109/ab109211/Images/ab109211-6-anti-sca1-ly6a-e-antibody-epr3355-immunohistochemistry-kidney-mouse.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Sca1 / Ly6A/E antibody [EPR3355]
See all Sca1 / Ly6A/E primary antibodies -
Description
Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse bone marrow, and Hepa1-6 lysates. IHC-P: mouse kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3355 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109211 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
|
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| IHC-P | (1) |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100 |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa). |
|
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. See IHC antigen retrieval protocols. For unpurified use at 1/50 - 1/100 |
Target
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Function
T-cell activation. -
Tissue specificity
Widely expressed. -
Sequence similarities
Contains 1 UPAR/Ly6 domain. -
Post-translational
modificationsO-glycosylated. Not N-glycosylated.
Not phosphorylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 110454 Mouse
- SwissProt: P05533 Mouse
- Unigene: 425949 Mouse
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Alternative names
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Sca1 / Ly6A/E with Purified ab109211 at 1:150 dilution (1.37 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)](https://www.abcam.com/ps/products/109/ab109211/Images/ab109211-367033-anti-sca1-ly6a-e-antibody-epr3355-westernblot-hepa16-mouse.jpg)
Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/10000 dilution (Purified) + Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)](https://www.abcam.com/ps/products/109/ab109211/Images/ab109211-367032-anti-sca1-ly6a-e-antibody-epr3355-westernblot-bone-marrow-mouse.jpg)
Western blot - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/1000 dilution (Purified) + Mouse bone marrow lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)](https://www.abcam.com/ps/products/109/ab109211/Images/ab109211-121838-anti-sca1-ly6a-e-antibody-epr3355-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)](https://www.abcam.com/ps/products/109/ab109211/Images/ab109211-121837-anti-sca1-ly6a-e-antibody-epr3355-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)Image from Basile LA et al., PLoS One. 2012;7(2):e30434. Epub 2012 Feb 24. Fig 5.; doi:10.1371/journal.pone.0030434; February 24, 2012, PLoS ONE 7(2): e30434.Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)](https://www.abcam.com/ps/products/109/ab109211/Images/ab109211-7-benefits-of-recombinant-antibodies.png)
Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab109211 has been referenced in 10 publications.
- Xie BS et al. Apolipoprotein E, low-density lipoprotein receptor, and immune cells control blood-brain barrier penetration by AAV-PHP.eB in mice. Theranostics 11:1177-1191 (2021). PubMed: 33391529
- Zhu D et al. Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair. Nat Commun 12:1412 (2021). PubMed: 33658506
- Batista AR et al. Ly6a Differential Expression in Blood-Brain Barrier Is Responsible for Strain Specific Central Nervous System Transduction Profile of AAV-PHP.B. Hum Gene Ther 31:90-102 (2020). PubMed: 31696742
- Silva-Pinheiro P et al. A Single Intravenous Injection of AAV-PHP.B-hNDUFS4 Ameliorates the Phenotype of Ndufs4-/- Mice. Mol Ther Methods Clin Dev 17:1071-1078 (2020). PubMed: 32478122
- Lu H et al. Liver-specific knockout of histone methyltransferase G9a impairs liver maturation and dysregulates inflammatory, cytoprotective, and drug-processing genes. Xenobiotica 49:740-752 (2019). PubMed: 29912608
- Tal A et al. Characterization of cell fusion in an experimental mouse model of endometriosis†. Biol Reprod 100:390-397 (2019). PubMed: 30304517
- Natale BV et al. Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta. Sci Rep 7:5575 (2017). PubMed: 28717241
- Passaro D et al. Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia. Cancer Cell 32:324-341.e6 (2017). PubMed: 28870739
- Okamoto M et al. Stem Cell Antigen 1-Positive Mesenchymal Cells Are the Origin of Follicular Cells during Thyroid Regeneration. PLoS One 8:e80801 (2013). IHC-P ; Mouse . PubMed: 24278321
- Basile LA et al. HemaMax™, a recombinant human interleukin-12, is a potent mitigator of acute radiation injury in mice and non-human primates. PLoS One 7:e30434 (2012). IHC-P ; Mouse . PubMed: 22383962