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    sca1--ly6ae-antibody-epr3355-bsa-and-azide-free-ab239951.pdf

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RecombinantRabMAb

Recombinant Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)
  • Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Unconjugated

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Overview

  • Product name

    Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free
    See all Sca1 / Ly6A/E primary antibodies
  • Description

    Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: mouse kidney tissue.
  • General notes

    ab239951 is the carrier-free version of ab109211.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3355
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Stem cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development

Associated products

  • Alternative Versions

    • Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunohistochemistry kits

    • Rabbit specific HRP/DAB Detection IHC Detection Kit - Micro-polymer (ab236469)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab239951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

 

WB
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

 

WB
Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    T-cell activation.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 UPAR/Ly6 domain.
  • Post-translational
    modifications

    O-glycosylated. Not N-glycosylated.
    Not phosphorylated.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P05533 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 110454 Mouse
    • SwissProt: P05533 Mouse
    • Unigene: 425949 Mouse
    • Alternative names

      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Sca1 / Ly6A/E with Purified ab109211 at 1:150 dilution (1.37 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109211)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)

      ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109211).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)Image from Basile LA et al. PLoS One. 2012;7(2):e30434. Epub 2012 Feb 24. Fig 5.; doi:10.1371/journal.pone.0030434; February 24; 2012; PLoS ONE 7(2): e30434.

      Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109211).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)
      Anti-Sca1 / Ly6A/E antibody [EPR3355] - BSA and Azide free (ab239951)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab239951? Please let us know so that we can cite the reference in this datasheet.

    ab239951 has not yet been referenced specifically in any publications.

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