Product nameAnti-Sca1 / Ly6A/E antibody - Hematopoietic Stem Cell Marker
See all Sca1 / Ly6A/E primary antibodies
DescriptionRabbit polyclonal to Sca1 / Ly6A/E - Hematopoietic Stem Cell Marker
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Sca1/ Ly6A/E aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse thymus and kidney tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).|
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 UPAR/Ly6 domain.
modificationsO-glycosylated. Not N-glycosylated.
Cellular localizationCell membrane.
- Information by UniProt
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
All lanes : Anti-Sca1 / Ly6A/E antibody - Hematopoietic Stem Cell Marker (ab93843) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The band observed at 13 kDa could potentially be a cleaved form of Sca1/Ly6A/E due to the presence of a 26 amino acid signal peptide.
ab93843 has not yet been referenced specifically in any publications.