Overview

  • Product name

    Anti-Sca1 / Ly6A/E antibody
    See all Sca1 / Ly6A/E primary antibodies
  • Description

    Rabbit polyclonal to Sca1 / Ly6A/E
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Sca1/ Ly6A/E aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab104951)

  • Positive control

    • This antibody gave a positive signal in Mouse thymus and kidney tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab93843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).

Target

  • Function

    T-cell activation.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Contains 1 UPAR/Ly6 domain.
  • Post-translational
    modifications

    O-glycosylated. Not N-glycosylated.
    Not phosphorylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ly-6A.2/Ly-6E. antibody
    • Ly-6A.2/Ly-6E.1 antibody
    • Ly6a antibody
    • LY6A_MOUSE antibody
    • Ly6al antibody
    • Lymphocyte antigen 6A-2/6E-1 antibody
    • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
    • SCA-1 antibody
    • Stem cell antigen 1 antibody
    • T cell activating protein antibody
    • T-cell-activating protein antibody
    • TAP antibody
    see all

Images

  • All lanes : Anti-Sca1 / Ly6A/E antibody (ab93843) at 1 µg/ml

    Lane 1 : Thymus (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 14 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 31 kDa, 51 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    The band observed at 13 kDa could potentially be a cleaved form of Sca1/Ly6A/E due to the presence of a 26 amino acid signal peptide.

References

ab93843 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab93843.
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