Product nameAnti-Sca1 / Ly6A/E antibody
See all Sca1 / Ly6A/E primary antibodies
DescriptionRabbit polyclonal to Sca1 / Ly6A/E
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse Sca1/ Ly6A/E aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Mouse thymus and kidney tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 14 kDa).|
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 UPAR/Ly6 domain.
modificationsO-glycosylated. Not N-glycosylated.
Cellular localizationCell membrane.
- Information by UniProt
- Ly-6A.2/Ly-6E. antibody
- Ly-6A.2/Ly-6E.1 antibody
- Ly6a antibody
All lanes : Anti-Sca1 / Ly6A/E antibody (ab93843) at 1 µg/ml
Lane 1 : Thymus (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Additional bands at: 31 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The band observed at 13 kDa could potentially be a cleaved form of Sca1/Ly6A/E due to the presence of a 26 amino acid signal peptide.
ab93843 has not yet been referenced specifically in any publications.