• Product name
    Anti-Sca1 / Ly6A/E antibody [D7] (FITC)
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [D7] to Sca1 / Ly6A/E (FITC)
  • Host species
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody is specific for Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-Fr, Cellular Activation, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse Sca1/ Ly6A/E. IL2 dependent T cell line CTL-L.

  • Positive control
    • ICC/IF: MEF cells. Flow Cyt: Con-A stimulated C57BL/6 mouse splenocytes.



Our Abpromise guarantee covers the use of ab25031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
IHC-Fr Use at an assay dependent concentration.
Cellular Activation Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21858462


  • Function
    T-cell activation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 1 UPAR/Ly6 domain.
  • Post-translational
    O-glycosylated. Not N-glycosylated.
    Not phosphorylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ly-6A.2/Ly-6E. antibody
    • Ly-6A.2/Ly-6E.1 antibody
    • Ly6a antibody
    • LY6A_MOUSE antibody
    • Ly6al antibody
    • Lymphocyte antigen 6A-2/6E-1 antibody
    • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
    • SCA-1 antibody
    • Stem cell antigen 1 antibody
    • T cell activating protein antibody
    • T-cell-activating protein antibody
    • TAP antibody
    see all


  • Flow Cytometry analysis of Con-A stimulated C57BL/6 mouse splenocytes labeling Ly-6A with ab25031 at 3 μg/106 cells dilution (purple). Grey - Isotype Control, Rat IgG2a-FITC.

  • BALB/c spleen cells were activated by incubation for 48 hours with concanavalin A. After harvesting, the cells were stained with Rat anti-mouse Ly6A/E-FITC (less than or equal to 0.5 ug/106 cells) and activated cells were gated and analyzed on a FACScan flow cytometer.
  • ICC/IF image of ab25031 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25031, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Liu H  et al. Negative pressure wound therapy promotes muscle-derived stem cell osteogenic differentiation through MAPK pathway. J Cell Mol Med 22:511-520 (2018). Flow Cyt ; Rat . Read more (PubMed: 28944996) »
  • He ZQ  et al. Generation of Mouse Haploid Somatic Cells by Small Molecules for Genome-wide Genetic Screening. Cell Rep 20:2227-2237 (2017). Read more (PubMed: 28854370) »
See all 14 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Flow Cytometry
None, cells were used fresh
Mouse Cell (Cells from bone marrow)
Cells from bone marrow
Gating Strategy
Alive cells (DAPI-), blood cells exluded
Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM

Dr. Francois Daubeuf

Verified customer

Submitted Feb 24 2015

Immunocytochemistry/ Immunofluorescence
Mouse Cell (Bone marrow)
Bone marrow
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 09 2012


Thank you for contacting us. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. I found conflicting information about the expression of CD90 on NIH3T3 cells: No expression or expression of Thy1.2 (CD90.2). ab226 only recognizes Thy1.1 (CD90.1). I therefore suggest to use ab25672 (Anti-CD90 / Thy1 antibody [G7] (FITC)) which will bind CD90.1 and CD90.2. I could not find any reliable data on the expression of Ly6 on NIH3T3 cells either, but can confirm that ab25031 will bind to Ly6A.2 and Ly6E. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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