Flow Cytometry abreview for Anti-Sca1 / Ly6A/E antibody [D7] (FITC)

Excellent
Abreviews
Application
Flow Cytometry
Fixation
None, cells were used fresh
Permeabilization
No
Sample
Mouse Cell (Cells from bone marrow)
Specification
Cells from bone marrow
Gating Strategy
Alive cells (DAPI-), blood cells exluded
Preparation
Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM

Other product details

Incubation time
30 minute(s) · Temperature: 20°C · Diluent: PBS, BSA 2%, EDTA 3mM
Dilution
1/100

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
After incubation with Sca1/Ly6A antibody, cells were centrifuged (400g, 5min), rinced with PBS-BSA 2%, centrifuged (400g, 5min) then DAPI was added 20min prior to count cells. Black dots represent isotype control and Green dots are Sca1/Ly6A+ cells

Dr. Francois Daubeuf

Verified customer

Submitted Feb 24 2015

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