After incubation with Sca1/Ly6A antibody, cells were centrifuged (400g, 5min), rinced with PBS-BSA 2%, centrifuged (400g, 5min) then DAPI was added 20min prior to count cells.
Black dots represent isotype control and Green dots are Sca1/Ly6A+ cells
Dr. Francois Daubeuf
Submitted Feb 24 2015
Get resources and offers direct to your inboxSign up