Key features and details
- Rabbit polyclonal to SCA10
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-SCA10 antibody
See all SCA10 primary antibodies
DescriptionRabbit polyclonal to SCA10
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Cynomolgus monkey, Orangutan
Recombinant fragment (His-T7-tag) corresponding to Human SCA10 aa 116-302. Expressed in E.coli.
LLFRELRVEQESLLTAFRCGLQFLGNIASRNEDSQSIVWVHAFPELFLSC LNHPDKKIVAYSSMILFTSLNHERMKELEENLNIAIDVIDAYQKHPESEW PFLIITDLFLKSPELVQAMFPKLNNQERVTLLDLMIAKITSDEPLTKDDI PVFLRHAELIASTFVDQCKTVLKLASEEPPDDEEALA
Database link: Q9UBB4
- WB: Recombinant human SCA10 protein; HEK-293T and HeLa cell lysates; pig kidney and brain lysates. IHC-P: Human prostate, prostate cancer, liver, liver cancer and kidney tissue. ICC/IF: HeLa cells.
This product was previously labelled as ATXN10
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 53 kDa.|
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
FunctionNecessary for the survival of cerebellar neurons (By similarity). Induces neuritogenesis by activating the Ras-MAP kinase pathway (By similarity). May play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.
Tissue specificityExpressed in the central nervous system.
Involvement in diseaseDefects in ATXN10 are the cause of spinocerebellar ataxia type 10 (SCA10) [MIM:603516]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA10 is an autosomal dominant cerebellar ataxia (ADCA).
Sequence similaritiesBelongs to the ataxin-10 family.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- Ataxin 10 antibody
- Ataxin-10 antibody
- ATX10_HUMAN antibody
Anti-SCA10 antibody (ab231644) at 1 µg + Recombinant human SCA10 protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 53 kDa
All lanes : Anti-SCA10 antibody (ab231644) at 1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Pig kidney tissue lysate
Lane 4 : Pig brain tissue lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 53 kDa
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SCA10 (green) using ab231644 at 20 μg/mL in ICC/IF. FITC secondary antibody.
Formalin-fixed, paraffin-embedded human prostate tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human kidney tissue stained for SCA10 using ab231644 at 20 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231644 has not yet been referenced specifically in any publications.