Overview

  • Product name

    Anti-SCA10 antibody - C-terminal
    See all SCA10 primary antibodies
  • Description

    Rabbit polyclonal to SCA10 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide within Human SCA10 aa 425-475 (C terminal). The exact sequence is proprietary.
    Sequence:

    LTEDNSQNQDLIAKMEEQGLADASLLKKVGFEVEKKGEKLILKSTRDTPK P


    Database link: Q9UBB4

  • Positive control

    • HeLa and 293T cell lysate
  • General notes

     This product was previously labelled as ATXN10

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Necessary for the survival of cerebellar neurons (By similarity). Induces neuritogenesis by activating the Ras-MAP kinase pathway (By similarity). May play a role in the maintenance of a critical intracellular glycosylation level and homeostasis.
  • Tissue specificity

    Expressed in the central nervous system.
  • Involvement in disease

    Defects in ATXN10 are the cause of spinocerebellar ataxia type 10 (SCA10) [MIM:603516]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA10 is an autosomal dominant cerebellar ataxia (ADCA).
  • Sequence similarities

    Belongs to the ataxin-10 family.
  • Cellular localization

    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ataxin 10 antibody
    • Ataxin-10 antibody
    • ATX10_HUMAN antibody
    • Atxn10 antibody
    • Brain protein E46 homolog antibody
    • E46L antibody
    • FLJ37990 antibody
    • HUMEEP antibody
    • Like mouse brain protein E46 antibody
    • OTTHUMP00000197383 antibody
    • SCA10 antibody
    • Spinocerebellar ataxia 10 antibody
    • Spinocerebellar ataxia type 10 protein antibody
    see all

Images

  • All lanes : Anti-SCA10 antibody - C-terminal (ab199824) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
  • Immunoprecipitation analysis of HeLa cell lysate (1.0 mg per IP reaction; 20% of IP loaded) labeling SCA10 using ab199824 at 3 μg per reaction (lane 1). A Control IgG was used in lane 2. For blotting immunoprecipitated SCA10, ab199824 was used at 0.1 μg/ml.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab199824 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199824.
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