Key features and details
- Rabbit polyclonal to SCA2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SCA2 antibody
See all SCA2 primary antibodies
DescriptionRabbit polyclonal to SCA2
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment within Human SCA2 (internal sequence). The exact sequence is proprietary.
Database link: Q99700
- WB: A431 whole cell extract. ICC/IF: HCT 116 cells.
This product was previously labelled as ATX2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 140 kDa.|
FunctionInvolved in EGFR trafficking, acting as negative regulator of endocytic EGFR internalization at the plasma membrane.
Tissue specificityExpressed in the brain, heart, liver, skeletal muscle, pancreas and placenta. Isoform 1 is predominant in the brain and spinal cord. Isoform 4 is more abundant in the cerebellum. In the brain, broadly expressed in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.
Involvement in diseaseSpinocerebellar ataxia 2
Amyotrophic lateral sclerosis 13
Sequence similaritiesBelongs to the ataxin-2 family.
- Information by UniProt
- ASL13 antibody
- Ataxin 2 antibody
- Ataxin-2 antibody
Anti-SCA2 antibody (ab227915) at 1/500 dilution + A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg
Predicted band size: 140 kDa
5% SDS-PAGE gel.
HCT 116 (human colorectal carcinoma cell line) cells stained for SCA2 (green) using ab227915 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227915 has not yet been referenced specifically in any publications.