Anti-SCAMP1 antibody (ab3430)

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Western blot - Anti-SCAMP1 antibody (ab3430)
  • Western blot - Anti-SCAMP1 antibody (ab3430)

Key features and details

  • Rabbit polyclonal to SCAMP1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-SCAMP1 antibody

  • Description

    Rabbit polyclonal to SCAMP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig

  • Immunogen

    Synthetic peptide corresponding to Human SCAMP1 aa 1-17.
    Sequence:

    MSDFDSNPFADPDLNNP


    (Peptide available as ab4969)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: U-87MG, Neuro-2a, SH-SY5Y, SK-N-AS, T98G, IMR-32 and Rat brain tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).

By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).

By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate.

Target

Images

  • Western blot - Anti-SCAMP1 antibody (ab3430)
    Western blot - Anti-SCAMP1 antibody (ab3430)
    All lanes : Anti-SCAMP1 antibody (ab3430) at 2 µg/ml

    Lane 1 : U-87MG (Human primary glioblastoma cell line) lysate
    Lane 2 : Neuro-2a (Mouse brain) lysate
    Lane 3 : SH-SY5Y (Human derived cell line) lysate
    Lane 4 : SK-N-AS (Human neuroblastoma cell line) lysate
    Lane 5 : T98G (Glioblastoma cell line) lysate
    Lane 6 : IMR-32 (Human neuroblastoma cell line) lysate
    Lane 7 : Rat brain tissue

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution

    Predicted band size: 38 kDa
    Observed band size: 36 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% Milk.

  • Western blot - Anti-SCAMP1 antibody (ab3430)
    Western blot - Anti-SCAMP1 antibody (ab3430)

    Western blot detection of SCAMP1 on rat brain extract using ab3430.

     

References (2)

Publishing research using ab3430? Please let us know so that we can cite the reference in this datasheet.

ab3430 has been referenced in 2 publications.

  • Gerlini R  et al. Double knock-out of Hmga1 and Hipk2 genes causes perinatal death associated to respiratory distress and thyroid abnormalities in mice. Cell Death Dis 10:747 (2019). PubMed: 31582725
  • Jain P  et al. Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem 282:34581-93 (2007). WB, Dot blot ; Hamster . PubMed: 17897946

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