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    scamp1-antibody-ab3430.pdf

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Anti-SCAMP1 antibody (ab3430)

  • Datasheet
  • SDS
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Western blot - Anti-SCAMP1 antibody (ab3430)

    Key features and details

    • Rabbit polyclonal to SCAMP1
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-SCAMP1 antibody
    • Description

      Rabbit polyclonal to SCAMP1
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human SCAMP1 aa 1-17.
      Sequence:

      MSDFDSNPFADPDLNNP


      (Peptide available as ab4969)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurotransmission
      • Secretory Vesicles
      • Other
      • Signal Transduction
      • Protein Trafficking
      • Organelle Proteins

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3430 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Rat
    All applications
    Pig
    Application Abreviews Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).

    By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate.

    Notes
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 38 kDa).

    By Western blot, this antibody detects an ~31 kDa protein representing SCAMP 1 rat brain homogenate.

    Target

    • Function

      Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.
    • Tissue specificity

      Widely expressed, with highest expression in brain.
    • Sequence similarities

      Belongs to the SCAMP family.
    • Cellular localization

      Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane.
    • Target information above from: UniProt accession O15126 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 397477 Pig
      • Entrez Gene: 29521 Rat
      • SwissProt: O77735 Pig
      • SwissProt: P56603 Rat
      • Unigene: 20374 Rat
      • Alternative names

        • SCAM1_HUMAN antibody
        • SCAMP 1 antibody
        • SCAMP 37 antibody
        • SCAMP antibody
        • SCAMP1 antibody
        • SCAMP37 antibody
        • Secretory carrier associated membrane protein 1 antibody
        • Secretory carrier membrane protein 1 antibody
        • Secretory carrier membrane protein antibody
        • Secretory carrier-associated membrane protein 1 antibody
        see all

      Images

      • Western blot - Anti-SCAMP1 antibody (ab3430)
        Western blot - Anti-SCAMP1 antibody (ab3430)

        Western blot detection of SCAMP1 on rat brain extract using ab3430.

         

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab3430? Please let us know so that we can cite the reference in this datasheet.

      ab3430 has been referenced in 1 publication.

      • Jain P  et al. Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein. J Biol Chem 282:34581-93 (2007). WB, Dot blot ; Hamster . PubMed: 17897946

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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