Recombinant
RabMAb

Anti-SCAMP1 antibody [EPR14493(B)] (ab185951)

Overview

  • Product name
    Anti-SCAMP1 antibody [EPR14493(B)]
    See all SCAMP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14493(B)] to SCAMP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SCAMP1 aa 50-150. The exact sequence is proprietary.
    Database link: O15126

  • Positive control
    • U87-MG, HeLa, Neuro-2a, Human fetal brain, C6 and RAW 264.7 lysates; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

Images

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SCAMP1 with ab185951 at 1/160 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • All lanes : Anti-SCAMP1 antibody [EPR14493(B)] (ab185951) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Neuro-2a cell lysate
    Lane 4 : Human fetal brain cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

  • All lanes : Anti-SCAMP1 antibody [EPR14493(B)] (ab185951) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa

References

ab185951 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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