Overview

  • Product name

    Anti-SCAMP2/SC2 antibody [EPR10185]
    See all SCAMP2/SC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10185] to SCAMP2/SC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SCAMP2/SC2. The exact sequence is proprietary.
    Database link: O15127

  • Positive control

    • HepG2, 293T and Human tonsil lysates, Human pancreas tissue, permeabilized 293T cells
  • General notes

     

     

     This product was previously labelled as SCAMP2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the SCAMP family.
  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • SCAM2_HUMAN antibody
    • SCAMP2 antibody
    • Secretory carrier membrane protein 2 antibody
    • Secretory carrier-associated membrane protein 2 antibody

Images

  • All lanes : Anti-SCAMP2/SC2 antibody [EPR10185] (ab154181) at 1/1000 dilution

    Lane 1 : HepG2 lysates
    Lane 2 : 293T lysates
    Lane 3 : Human tonsil lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 37 kDa

  • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SCAMP2/SC2 with ab154181 at 1/250 dilution.

  • Flow cytometric analysis of permeabilized 293T cells with ab154181 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
  • Immunohistochemical analysis of paraffin embedded Human prostate hyperplasia tissue using ab154181 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154181 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue using ab154181 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154181 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab154181 showing +ve staining.

References

ab154181 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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