Anti-SCAMP3/SC3 antibody (ab219385)
Key features and details
- Rabbit polyclonal to SCAMP3/SC3
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCAMP3/SC3 antibody
See all SCAMP3/SC3 primary antibodies -
Description
Rabbit polyclonal to SCAMP3/SC3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment corresponding to Human SCAMP3/SC3 aa 1-200.
Database link: O14828 -
Positive control
- Human adrenal tissue; HepG2 cell; EC109 and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Caprylic Acid - Ammonium Sulfate precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml.
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ICC/IF |
1/100.
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 27, 38 kDa (predicted molecular weight: 38 kDa).
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. |
ICC/IF
1/100. |
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 27, 38 kDa (predicted molecular weight: 38 kDa). |
Target
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Function
Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface. -
Tissue specificity
Widely expressed, with highest expression in heart and skeletal muscle. -
Sequence similarities
Belongs to the SCAMP family. -
Post-translational
modificationsMonoubiquitinated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 539670 Cow
- Entrez Gene: 10067 Human
- Entrez Gene: 24045 Mouse
- Omim: 606913 Human
- SwissProt: Q58DR5 Cow
- SwissProt: O14828 Human
- SwissProt: O35609 Mouse
- Unigene: 200600 Human
see all -
Alternative names
- C1orf3 antibody
- Propin 1 antibody
- Propin1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human adrenal tissue labeling SCAMP3/SC3 with ab219385 at 10 µg/mL.
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All lanes : Anti-SCAMP3/SC3 antibody (ab219385) at 2 µg/ml
Lane 1 : EC109 whole cell lysate
Lane 2 : 293T whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 38 kDa -
Immunofluorescent analysis of HepG2 cells labeling SCAMP3/SC3 with ab219385 at 1/100 dilution. Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) was used as a secondary.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab219385 has not yet been referenced specifically in any publications.