Key features and details
- Rabbit polyclonal to SCAMP5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SCAMP5 antibody
See all SCAMP5 primary antibodies
DescriptionRabbit polyclonal to SCAMP5
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
- IHC-P: Human cerebral cortex tissue. WB: SCAMP5 overexpression HEK-293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 36 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRequired for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.
Tissue specificityExpressed both by neuronal and nonneuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculate of the cortex region (at protein level).
Sequence similaritiesBelongs to the SCAMP family. SCAMP5 subfamily.
Cellular localizationCell membrane. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles.
- Information by UniProt
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Paraffin-embedded human liver tissue stained for SCAMP5 using ab254900 at 1/50 dilution in immunohistochemical analysis. Low expression as expected.
Paraffin-embedded human cerebral cortex tissue stained for SCAMP5 using ab254900 at 1/50 dilution in immunohistochemical analysis.
All lanes : Anti-SCAMP5 antibody (ab254900) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cell lysate
Lane 2 : SCAMP5 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 36 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254900 has not yet been referenced specifically in any publications.