Anti-SCAMP5 antibody (ab3432)
Key features and details
- Rabbit polyclonal to SCAMP5
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-SCAMP5 antibody
See all SCAMP5 primary antibodies -
Description
Rabbit polyclonal to SCAMP5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Rat SCAMP5 aa 217-233.
Sequence:NQPQTQYSATPNYTYSN
(Peptide available asab4971) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.
|
Notes |
---|
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa. |
Target
-
Function
Required for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway. -
Tissue specificity
Expressed both by neuronal and nonneuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculate of the cortex region (at protein level). -
Sequence similarities
Belongs to the SCAMP family. SCAMP5 subfamily. -
Cellular localization
Cell membrane. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles. - Information by UniProt
-
Database links
- Entrez Gene: 192683 Human
- Entrez Gene: 56807 Mouse
- Entrez Gene: 65171 Rat
- Omim: 613766 Human
- SwissProt: Q8TAC9 Human
- SwissProt: Q9JKD3 Mouse
- SwissProt: Q9JKE3 Rat
- Unigene: 374180 Human
see all -
Alternative names
- AI426171 antibody
- AW558254 antibody
- DKFZp686L1799 antibody
see all
Images
-
Anti-SCAMP5 antibody (ab3432) + Rat brain extract
Observed band size: 25 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab3432 has been referenced in 3 publications.
- Gong T et al. Identification of immune-related endoplasmic reticulum stress genes in sepsis using bioinformatics and machine learning. Front Immunol 13:995974 (2022). PubMed: 36203606
- Yang Y et al. Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of a-synuclein via exosome. PLoS One 12:e0180892 (2017). WB ; Rat, Human . PubMed: 28700687
- Noh JY et al. SCAMP5 Links Endoplasmic Reticulum Stress to the Accumulation of Expanded Polyglutamine Protein Aggregates via Endocytosis Inhibition. J Biol Chem 284:11318-25 (2009). ICC/IF . PubMed: 19240033