Overview

  • Product name

  • Description

    Rabbit polyclonal to SCAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 450-500 of Human SCAP (NP_036367.2).

  • Positive control

    • HeLa, Jurkat and 293T whole cell lysates
  • General notes

    Shelf Life: 1 year from date of receipt

Properties

Applications

Our Abpromise guarantee covers the use of ab125186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 140 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.
  • Sequence similarities

    Belongs to the WD repeat SCAP family.
    Contains 1 SSD (sterol-sensing) domain.
    Contains 7 WD repeats.
  • Domain

    Cholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0199 antibody
    • SCAP antibody
    • SCAP_HUMAN antibody
    • SREBF chaperone antibody
    • SREBF chaperone protein antibody
    • SREBP cleavage activating protein antibody
    • SREBP cleavage-activating protein antibody
    • Sterol regulatory element binding protein cleavage-activating protein antibody
    • Sterol regulatory element-binding protein cleavage-activating protein antibody
    see all

Images

  • All lanes : Anti-SCAP antibody (ab125186) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 140 kDa


    Exposure time: 30 seconds
  • ab125186, at 6 µg/mg lysate, detecting SCAP in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
    For the subsequent Western blot, ab125186 was used at 1 µg/ml.
    The blot was developed using the ECL technique, with an exposure time of 30 seconds.

References

This product has been referenced in:

  • Benetti E  et al. Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice. PLoS One 13:e0189707 (2018). Read more (PubMed: 29342166) »
See 1 Publication for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse embryo fibroblasts)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Treatment
RNAi
Specification
mouse embryo fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Boyan Zhang

Verified customer

Submitted Aug 30 2018

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HuH7.5 (hepatocyte-derived carcinoma cell line))
Total protein in input
600 µg
Immuno-precipitation step
Protein A
Specification
HuH7.5 (hepatocyte-derived carcinoma cell line)

Abcam user community

Verified customer

Submitted Apr 25 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (HuH7.5 Cell line ( hepatocyte-derived carcinoma ce)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
100 µg
Specification
HuH7.5 Cell line ( hepatocyte-derived carcinoma ce
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 17 2017

Question
Answer

DISCOUNT CODE: ***
Expiration date: September 9, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab125186 using mouse or rat samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your reply and for the additional information.

I am sending a vial of ab86415 free of charge on the order ***. This antibody is in stock and should arrive on Monday. Please keep me updated about how this antibody works in your Western blots.

Since Ab125186 has not been tested in mouse or rat samples, I can offer a discount off a future purchase if you buy the antibodynow, test it in one of these speciesand submit feedback to us in the form of an Abreview. The discount would be worth1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab125186 in mouse or rat. I will then send a discount code.

2. Purchase ab125186 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse or rat.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab125186 turns out to be unsuitable for mouse or rat, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us and for letting us know about the trouble with ab86351. I am sorry to see that the results have been so poor.

I would be happy to send a vial of ab86415 as a replacement. Do you have your original order or PO number, so that I can arrange for this replacement?

For our records, I also have some additional questions about the protocol:

1) How much protein was loaded per lane of the gel?
2) What protease inhibitors were used, and was this a standard reduced and denatured Western blot?
3) What was used to block the membrane (i.e. milk or BSA)?
4) What dilution of antibody was used?

We only have full size vials of all of our products, so unfortunately I am not able to send samples ofthe SCAP antibodies ab125186 and ab91323. We do guarantee them both to work in Western blot with human samples, so if you had any trouble with them we would issue replacements, credits, or refunds. If you would be using them in an untested species (like mouse or rat) we do have a testing discount program, and I would be happy to send more information about that program if it's applicable.

I look forward to hearing from you and resolving this promptly. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

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