Key features and details
- Rabbit polyclonal to SCAP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SCAP antibody
See all SCAP primary antibodies
DescriptionRabbit polyclonal to SCAP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide, corresponding to a region within amino acids 450-500 of Human SCAP (NP_036367.2).
- HeLa, Jurkat and 293T whole cell lysates
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. The product can be stored for up to 12 months.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab125186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 140 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEscort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.
Sequence similaritiesBelongs to the WD repeat SCAP family.
Contains 1 SSD (sterol-sensing) domain.
Contains 7 WD repeats.
DomainCholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
- Information by UniProt
- KIAA0199 antibody
- SCAP antibody
- SCAP_HUMAN antibody
All lanes : Anti-SCAP antibody (ab125186) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 140 kDa
Exposure time: 30 seconds
ab125186, at 6 µg/mg lysate, detecting SCAP in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125186 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 30 seconds.
ab125186 has been referenced in 2 publications.
- Pagano S et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation. J Clin Med 8:N/A (2019). PubMed: 31766415
- Benetti E et al. Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice. PLoS One 13:e0189707 (2018). PubMed: 29342166