Anti-SCAP antibody (ab125186)
Key features and details
- Rabbit polyclonal to SCAP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SCAP antibody
See all SCAP primary antibodies -
Description
Rabbit polyclonal to SCAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 450-500 of Human SCAP (NP_036367.2).
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Positive control
- HeLa, Jurkat and 293T whole cell lysates
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab125186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 140 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. -
Sequence similarities
Belongs to the WD repeat SCAP family.
Contains 1 SSD (sterol-sensing) domain.
Contains 7 WD repeats. -
Domain
Cholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
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Database links
- Entrez Gene: 22937 Human
- Omim: 601510 Human
- SwissProt: Q12770 Human
- Unigene: 531789 Human
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Alternative names
- KIAA0199 antibody
- SCAP antibody
- SCAP_HUMAN antibody
see all
Images
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Western blot - Anti-SCAP antibody (ab125186)All lanes : Anti-SCAP antibody (ab125186) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 140 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-SCAP antibody (ab125186)ab125186, at 6 µg/mg lysate, detecting SCAP in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125186 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 30 seconds.
Protocols
Datasheets and documents
References (2)
ab125186 has been referenced in 2 publications.
- Pagano S et al. Anti-ApoA-1 IgGs in Familial Hypercholesterolemia Display Paradoxical Associations with Lipid Profile and Promote Foam Cell Formation. J Clin Med 8:N/A (2019). PubMed: 31766415
- Benetti E et al. Effects of vitamin D on insulin resistance and myosteatosis in diet-induced obese mice. PLoS One 13:e0189707 (2018). PubMed: 29342166
