Overview

  • Product name

    Anti-SCAP antibody - C-terminal
    See all SCAP primary antibodies
  • Description

    Rabbit polyclonal to SCAP - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 977-1224 of Human SCAP (Uniprot ID: Q12770).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab153933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 140 kDa.

Target

  • Function

    Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.
  • Sequence similarities

    Belongs to the WD repeat SCAP family.
    Contains 1 SSD (sterol-sensing) domain.
    Contains 7 WD repeats.
  • Domain

    Cholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA0199 antibody
    • SCAP antibody
    • SCAP_HUMAN antibody
    • SREBF chaperone antibody
    • SREBF chaperone protein antibody
    • SREBP cleavage activating protein antibody
    • SREBP cleavage-activating protein antibody
    • Sterol regulatory element binding protein cleavage-activating protein antibody
    • Sterol regulatory element-binding protein cleavage-activating protein antibody
    see all

Images

  • Anti-SCAP antibody - C-terminal (ab153933) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 140 kDa



    5% SDS PAGE

References

ab153933 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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