The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 57 kDa).
Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.
Belongs to the CD36 family.
Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
Western blot - Anti-Scavenging Receptor SR-BI antibody - C-terminal (ab193960)
Anti-Scavenging Receptor SR-BI antibody - C-terminal (ab193960) at 0.01 µg/ml + Human adrenal gland lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 1 hour
We do note that Scavenging Receptor SR-BI is a glycoprotein which makes a higher size band to be expected, and this is consistent with observations with different antibodies from other commercial sources.