Name: Anti-Scavenging Receptor SR-BI antibody [EP1556Y]
Brand: RabMAb
SKU: ab52629
Rating: 5 (4 reviews)

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Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free HRP

Product name

Anti-Scavenging Receptor SR-BI antibody [EP1556Y]
See all Scavenging Receptor SR-BI primary antibodies
Description

Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep
Immunogen

Synthetic peptide within Human Scavenging Receptor SR-BI aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q8WTV0
Positive control
- WB: Mouse liver tissue lysate. IHC-P: Human liver tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1556Y
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human Scavenging Receptor SR-BI protein (Fc Chimera) (ab182680)
Related Products
- Sodium salicylate, anti-inflammatory agent (ab120746)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(2)	1/1000 - 1/2000. Detects a band of approximately 80 kDa.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/1000 - 1/2000. Detects a band of approximately 80 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt or ICC.

Function

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.
Tissue specificity

Widely expressed.
Sequence similarities

Belongs to the CD36 family.
Post-translational
modifications

N-glycosylated.
Cellular localization

Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
Target information above from: UniProt accession Q8WTV0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 949 Human
- Entrez Gene: 20778 Mouse
- Entrez Gene: 25073 Rat
- Omim: 601040 Human
- SwissProt: Q8WTV0 Human
- SwissProt: Q61009 Mouse
- SwissProt: P97943 Rat
- Unigene: 520348 Human
- Unigene: 282242 Mouse
- Unigene: 88169 Rat
see all
Alternative names
- CD36 and LIMPII analogous 1 antibody
- CD36 antibody
- CD36 Antigen like 1 antibody
- CD36 antigen-like 1 antibody
- CD36L1 antibody
- CLA 1 antibody
- CLA-1 antibody
- CLA1 antibody
- Collagen type I receptor antibody
- HDLQTL6 antibody
- MGC138242 antibody
- SCARB1 antibody
- Scavebger Receptor Class B Member 1 antibody
- Scavenger receptor class B member 1 antibody
- Scavenger Receptor Class B Type 1 antibody
- SCRB1_HUMAN antibody
- SR BI antibody
- SR-BI antibody
- SRB1 antibody
- SRBI antibody
- Thrombospondin receptor like 1 antibody
- thrombospondin receptor-like 1 antibody
see all

Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : SCARB1 knockout HEK-293T cell lysate
Lane 3 : Human Liver cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Observed band size: 70,75 kDa why is the actual band size different from the predicted?

False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [EP1556Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52629 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70/75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line ab282646 (knockout cell lysate ab283046). To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Human liver whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab52629 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab52629 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Observed band size: 80 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 lysate
Lane 3 : PC-3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Observed band size: 80 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/2000 dilution (unpurified) + Mouse liver lysate at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Observed band size: 80 kDa why is the actual band size different from the predicted?
Western blot - Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

THP1 cells were incubated at 37ºC for 40h with vehicle control (0 µM) and different concentrations of sodium salicylate (ab120746). Decreased expression of scavenging receptor SR-BI in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab52629 at 1/2000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab52629? Please let us know so that we can cite the reference in this datasheet.

ab52629 has been referenced in 45 publications.

Shi W et al. Reduction of TMAO level enhances the stability of carotid atherosclerotic plaque through promoting macrophage M2 polarization and efferocytosis. Biosci Rep 41:N/A (2021). PubMed: 33969376
Jiang T et al. SR-B1 and CD10 combined immunoprofile for differential diagnosis of metastatic clear cell renal cell carcinoma and clear cell carcinoma of the ovary. J Mol Histol 52:539-544 (2021). PubMed: 33608777
Du Y et al. Butyrate protects against high-fat diet-induced atherosclerosis via up-regulating ABCA1 expression in apolipoprotein E-deficiency mice. Br J Pharmacol 177:1754-1772 (2020). PubMed: 31769014
Cruz W et al. Lipoprotein-Like Nanoparticle Carrying Small Interfering RNA Against Spalt-Like Transcription Factor 4 Effectively Targets Hepatocellular Carcinoma Cells and Decreases Tumor Burden. Hepatol Commun 4:769-782 (2020). PubMed: 32363325
Tonini C et al. Inhibition of Bromodomain and Extraterminal Domain (BET) Proteins by JQ1 Unravels a Novel Epigenetic Modulation to Control Lipid Homeostasis. Int J Mol Sci 21:N/A (2020). PubMed: 32075110

View all Publications for this product

Customer reviews and Q&As

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1-7 of 7 Abreviews or Q&A

Application

Western blot

Sample

Goat Cell lysate - whole cell (mammary epithelial)

Gel Running Conditions

Reduced Denaturing (10%)

Loading amount

80 µg

Specification

mammary epithelial

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Qamar Taban

Verified customer

Submitted Aug 28 2020

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Spleen)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

40 µg

Specification

Spleen

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 21 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HEK293T)

Permeabilization

Yes - 0.1% Triton X-100

Specification

HEK293T

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Jul 14 2017

Application

Flow Cytometry

Sample

Mouse Cell (macrophage cell line RAW267.4)

Permeabilization

Gating Strategy

macrophages

Specification

macrophage cell line RAW267.4

Preparation

Cell harvesting/tissue preparation method: by cell scraper
Sample buffer: 1x PBS

Fixation

Formaldehyde

Mr. Pijus Barman

Verified customer

Submitted May 17 2011

Question

Inquiry: loading 40ug cell lysat from BAEC, prepared 2 identical blots probing blot 2 (lower) with ab24603 as a replacement for ab3, dilution 1:1000 Result: antibody not specific blot 1 (upper blot) probing with ab52629 dilution 1:2000 - excellent antibody!

Abcam community

Verified customer

Asked on Dec 12 2012

Answer

EThank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody, particularly as the previous one you were using succesfully is no longer available.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and Cow, Mouse and Rat samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could also provide an image which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.

Order Details
Antibody code:

Problem
Choose: Non-specific band Multiple bands No signal or weak signal High background

Lot number

Purchase order number
or preferably Abcam order number:

General Information
Antibody storage conditions (temperature/reconstitution etc)

Description of the problem (high background, wrong band size, more bands, no band etc.)

Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)

Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)

Amount of protein loaded

Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)

Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)

Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

Detection method (ECL, ECLPlus etc.)

Positive and negative controls used (please specify)

Optimization attempts (problem solving)
How many times have you tried the Western?

Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No
e.g. are the background bands always in the same place?

What steps have you altered?

Additional Notes:

Image:
We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

Abcam Scientific Support

Answered on Dec 12 2012

Question

Phone call requesting information on the concentration of Anti-Scavenging Receptor SR-BI antibody (ab52629), lot number GR52658-2.

Abcam community

Verified customer

Asked on Mar 19 2012

Answer

Thank you for contacting us this morning.

I can now confirm that the concentration of Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), lot number GR52658-2 is0.223 mg/mL.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Abcam Scientific Support

Answered on Mar 19 2012

Question

Hi I would like to use the following antibody: srb1 (ab52629). Could you provide the blasting results of your sequence immunogen against Rattus Norvegicus? thank in advance best

Abcam community

Verified customer

Asked on Feb 17 2012

Answer

Thank you for contacting us. This product has an 85% homolgy between the immunogen and rat protein. The sequence is I have plaved a link to blastp for rat below: http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=blastn&BLAST_PROG_DEF=megaBlast&SHOW_DEFAULTS=on&BLAST_SPEC=OGP__10116__10621 I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Abcam Scientific Support

Answered on Feb 18 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (45)

Customer reviews and Q&As

Western blot abreview for Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

Western blot abreview for Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

Flow Cytometry abreview for Anti-Scavenging Receptor SR-BI antibody [EP1556Y]

Post-translational
modifications