Validated using a knockout cell line

Recombinant Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Knockout Tested Rabbit recombinant monoclonal Scavenging Receptor SR-BI antibody [EP1556Y]. Validated in WB, IHC and tested in Mouse, Rat, Sheep, Human. Cited in 24 publication(s).


  • Product name

    Anti-Scavenging Receptor SR-BI antibody [EP1556Y]
    See all Scavenging Receptor SR-BI primary antibodies
  • Description

    Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Synthetic peptide within Human Scavenging Receptor SR-BI aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q8WTV0

  • Positive control

    • WB: Mouse liver tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab52629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 80 kDa.
IHC-P 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function

      Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.
    • Tissue specificity

      Widely expressed.
    • Sequence similarities

      Belongs to the CD36 family.
    • Post-translational

    • Cellular localization

      Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD36 and LIMPII analogous 1 antibody
      • CD36 antibody
      • CD36 Antigen like 1 antibody
      • CD36 antigen-like 1 antibody
      • CD36L1 antibody
      • CLA 1 antibody
      • CLA-1 antibody
      • CLA1 antibody
      • Collagen type I receptor antibody
      • HDLQTL6 antibody
      • MGC138242 antibody
      • SCARB1 antibody
      • Scavebger Receptor Class B Member 1 antibody
      • Scavenger receptor class B member 1 antibody
      • Scavenger Receptor Class B Type 1 antibody
      • SCRB1_HUMAN antibody
      • SR BI antibody
      • SR-BI antibody
      • SRB1 antibody
      • SRBI antibody
      • Thrombospondin receptor like 1 antibody
      • thrombospondin receptor-like 1 antibody
      see all


    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Human liver whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab52629 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab52629 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

      Lane 1 : Mouse liver lysate
      Lane 2 : Rat liver lysate

      Lysates/proteins at 20 µg per lane.

      All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : PC-3 cell lysate

      Lysates/proteins at 20 µg per lane.

      All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Observed band size: 80 kDa why is the actual band size different from the predicted?

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/2000 dilution (unpurified) + Mouse liver lysate at 10 µg

      goat anti-rabbit HRP at 1/2000 dilution

      Observed band size: 80 kDa why is the actual band size different from the predicted?

    • THP1 cells were incubated at 37ºC for 40h with vehicle control (0 µM) and different concentrations of sodium salicylate (ab120746). Decreased expression of scavenging receptor SR-BI in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab52629 at 1/2000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.



    This product has been referenced in:

    • Dong B  et al. Activation of FXR by obeticholic acid induces hepatic gene expression of SR-BI through a novel mechanism of transcriptional synergy with the nuclear receptor LXR. Int J Mol Med 43:1927-1938 (2019). Read more (PubMed: 30896855) »
    • He D  et al. Apolipoprotein A-1 mimetic peptide 4F promotes endothelial repairing and compromises reendothelialization impaired by oxidized HDL through SR-B1. Redox Biol 15:228-242 (2018). WB ; Mouse . Read more (PubMed: 29277016) »
    See all 28 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Western blot
    Mouse Cell lysate - whole cell (Spleen)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    40 µg
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Sep 21 2017

    Immunocytochemistry/ Immunofluorescence
    Human Cell (HEK293T)
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Jul 14 2017


    EThank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody, particularly as the previous one you were using succesfully is no longer available.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB and Cow, Mouse and Rat samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    I would appreciate if you could also provide an image which would help us to assess the results.

    Thank you for your time and cooperation. We look forward to receiving the completed questionaire.

    Order Details
    Antibody code:

    Choose: Non-specific band Multiple bands No signal or weak signal High background

    Lot number

    Purchase order number
    or preferably Abcam order number:

    General Information
    Antibody storage conditions (temperature/reconstitution etc)

    Description of the problem (high background, wrong band size, more bands, no band etc.)

    Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)

    Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)

    Amount of protein loaded

    Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)

    Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)

    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

    Detection method (ECL, ECLPlus etc.)

    Positive and negative controls used (please specify)

    Optimization attempts (problem solving)
    How many times have you tried the Western?

    Have you run a "No Primary" control?
    Yes No

    Do you obtain the same results every time?
    Yes No
    e.g. are the background bands always in the same place?

    What steps have you altered?

    Additional Notes:

    We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asess the results.

    Read More


    Thank you for contacting us this morning.

    I can now confirm that the concentration of Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), lot number GR52658-2 is0.223 mg/mL.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More


    Thank you for contacting us. This product has an 85% homolgy between the immunogen and rat protein. The sequence is I have plaved a link to blastp for rat below: I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More
    This product is known to not work in this application or species.
    Flow Cytometry
    Mouse Cell (macrophage cell line RAW267.4)
    Gating Strategy
    macrophage cell line RAW267.4
    Cell harvesting/tissue preparation method: by cell scraper
    Sample buffer: 1x PBS

    Mr. Pijus Barman

    Verified customer

    Submitted May 17 2011

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