Recombinant HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
- Conjugation: HRP
Related conjugates and formulations
Overview
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Product name
HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y]
See all Scavenging Receptor SR-BI primary antibodies -
Description
HRP Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse liver tissue lysate. IHC-P: normal human liver tissue sections
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1556Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/2000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/2000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa). |
Target
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Function
Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the CD36 family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae. - Information by UniProt
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Database links
- Entrez Gene: 949 Human
- Entrez Gene: 20778 Mouse
- Entrez Gene: 25073 Rat
- Omim: 601040 Human
- SwissProt: Q8WTV0 Human
- SwissProt: Q61009 Mouse
- SwissProt: P97943 Rat
- Unigene: 520348 Human
see all -
Alternative names
- CD36 and LIMPII analogous 1 antibody
- CD36 antibody
- CD36 Antigen like 1 antibody
see all
Images
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All lanes : HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233) at 1/2000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SCARB1 (Scavenging Receptor SR-BI) knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 60 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesab206233 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in SCARB1 (Scavenging Receptor SR-BI) knockout cells. Wild-type and SCARB1 (Scavenging Receptor SR-BI) knockout samples were subjected to SDS-PAGE. Ab206233 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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IHC image of Scavenging Receptor SR-BI staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab206233, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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HRP Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab206233) at 1/2000 dilution + Liver (Mouse) Tissue Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab206233 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab206233 has not yet been referenced specifically in any publications.