Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (HRP) (ab206233)

Overview

  • Product name

    Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (HRP)
    See all Scavenging Receptor SR-BI primary antibodies
  • Description

    Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Scavenging Receptor SR-BI aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q8WTV0

  • Positive control

    • WB: Mouse liver tissue lysate. IHC-P: normal human liver tissue sections
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab206233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 76 kDa (predicted molecular weight: 60 kDa).

Target

  • Function

    Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the CD36 family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD36 and LIMPII analogous 1 antibody
    • CD36 antibody
    • CD36 Antigen like 1 antibody
    • CD36 antigen-like 1 antibody
    • CD36L1 antibody
    • CLA 1 antibody
    • CLA-1 antibody
    • CLA1 antibody
    • Collagen type I receptor antibody
    • HDLQTL6 antibody
    • MGC138242 antibody
    • SCARB1 antibody
    • Scavebger Receptor Class B Member 1 antibody
    • Scavenger receptor class B member 1 antibody
    • Scavenger Receptor Class B Type 1 antibody
    • SCRB1_HUMAN antibody
    • SR BI antibody
    • SR-BI antibody
    • SRB1 antibody
    • SRBI antibody
    • Thrombospondin receptor like 1 antibody
    • thrombospondin receptor-like 1 antibody
    see all

Images

  • All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (HRP) (ab206233) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : SCARB1 (Scavenging Receptor SR-BI) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 60 kDa
    Observed band size: 76 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    ab206233 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in SCARB1 (Scavenging Receptor SR-BI) knockout cells. Wild-type and SCARB1 (Scavenging Receptor SR-BI) knockout samples were subjected to SDS-PAGE. Ab206233 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.

  • IHC image of Scavenging Receptor SR-BI staining in a section of formalin-fixed paraffin-embedded normal human liver*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab206233, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (HRP) (ab206233) at 1/2000 dilution + Liver (Mouse) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 76 kDa why is the actual band size different from the predicted?


    Exposure time: 8 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab206233 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab206233 has not yet been referenced specifically in any publications.

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