Validated using a knockout cell line
Recombinant
RabMAb

Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free (ab176238)

Overview

  • Product name
    Anti-Scavenging Receptor SR-BI antibody [EP1556Y] - Low endotoxin, Azide free
    See all Scavenging Receptor SR-BI primary antibodies
  • Description
    Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI - Low endotoxin, Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the CD36 family.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD36 and LIMPII analogous 1 antibody
      • CD36 antibody
      • CD36 Antigen like 1 antibody
      • CD36 antigen-like 1 antibody
      • CD36L1 antibody
      • CLA 1 antibody
      • CLA-1 antibody
      • CLA1 antibody
      • Collagen type I receptor antibody
      • HDLQTL6 antibody
      • MGC138242 antibody
      • SCARB1 antibody
      • Scavebger Receptor Class B Member 1 antibody
      • Scavenger receptor class B member 1 antibody
      • Scavenger Receptor Class B Type 1 antibody
      • SCRB1_HUMAN antibody
      • SR BI antibody
      • SR-BI antibody
      • SRB1 antibody
      • SRBI antibody
      • Thrombospondin receptor like 1 antibody
      • thrombospondin receptor-like 1 antibody
      see all

    Images

    • Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52629).

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Human liver whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab52629 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab52629 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab52629 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176238).

    References

    ab176238 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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