Recombinant
RabMAb

Recombinant Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

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Overview

  • Product name

    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal
    See all Scavenging Receptor SRB2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12081] to Scavenging Receptor SRB2 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Scavenging Receptor SRB2 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q14108

  • Positive control

    • MCF7 and HeLa whole cell lysates; Human fetal kidney lysate; Human and Mouse liver tissues; MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab196651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/20000. Detects a band of approximately 89 kDa (predicted molecular weight: 54 kDa).

Target

  • Cellular localization

    Lysosomal membrane

  • Database links

    see all

  • Alternative names

    • 85 kDa lysosomal membrane sialoglycoprotein antibody
    • 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
    • AMRF antibody
    • CD36 antigen (collagen type I receptor thrombospondin receptor) like 2 antibody
    • CD36 antigen like 2 antibody
    • CD36L2 antibody
    • EPM4 antibody
    • HLGP85 antibody
    • LGP85 antibody
    • LIMP 2 antibody
    • LIMP II antibody
    • LIMPII antibody
    • Lysosomal integral membrane protein II antibody
    • Lysosome membrane protein 2 antibody
    • Lysosome membrane protein II antibody
    • SCARB2 antibody
    • Scavenger receptor class B member 2 antibody
    • SR BII antibody
    see all

Images

  • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    All lanes : Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 21389126, PMID: 22272359 and PMID: 23635510).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Scavenging Receptor SRB2 with ab196651 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on human liver tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 54 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID: 21389126, PMID: 22272359 and PMID: 23635510).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Scavenging Receptor SRB2 with ab196651 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Flow Cytometry - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
    Flow Cytometry - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

    Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Scavenging Receptor SRB2 with ab196651 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

Publishing research using ab196651? Please let us know so that we can cite the reference in this datasheet.

ab196651 has not yet been referenced specifically in any publications.

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