For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    scavenging-receptor-srb2-antibody-epr12081-c-terminal-ab196651.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
  • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
  • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
  • Flow Cytometry (Intracellular) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
  • Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12081] to Scavenging Receptor SRB2 - C-terminal
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-PIP5K3/PIKFYVE antibody (ab137907)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Knockout
Product image
Human SCARB2 knockout MCF7 cell line (ab274952)

View more associated products

Overview

  • Product name

    Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal
    See all Scavenging Receptor SRB2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12081] to Scavenging Receptor SRB2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7 and HeLa whole cell lysates; Human fetal kidney lysate; Human and Mouse liver tissues; MCF7 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12081
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipoproteins/Apolipoproteins
    • Lipoproteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Scavenging Receptor SRB2 antibody [EPR12081] - BSA and Azide free (ab251221)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab196651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/20000. Detects a band of approximately 89 kDa (predicted molecular weight: 54 kDa).
Notes
Flow Cyt (Intra)
1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/20000. Detects a band of approximately 89 kDa (predicted molecular weight: 54 kDa).

Target

  • Cellular localization

    Lysosomal membrane
  • Database links

    • Entrez Gene: 950 Human
    • Entrez Gene: 12492 Mouse
    • Entrez Gene: 117106 Rat
    • Omim: 602257 Human
    • SwissProt: Q14108 Human
    • SwissProt: O35114 Mouse
    • SwissProt: P27615 Rat
    • Alternative names

      • 85 kDa lysosomal membrane sialoglycoprotein antibody
      • 85 kDa lysosomal sialoglycoprotein scavenger receptor class B member 2 antibody
      • AMRF antibody
      • CD36 antigen (collagen type I receptor thrombospondin receptor) like 2 antibody
      • CD36 antigen like 2 antibody
      • CD36L2 antibody
      • EPM4 antibody
      • HLGP85 antibody
      • LGP85 antibody
      • LIMP 2 antibody
      • LIMP II antibody
      • LIMPII antibody
      • Lysosomal integral membrane protein II antibody
      • Lysosome membrane protein 2 antibody
      • Lysosome membrane protein II antibody
      • SCARB2 antibody
      • Scavenger receptor class B member 2 antibody
      • SR BII antibody
      see all

    Images

    • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      All lanes : Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution

      Lane 1 : Wild-type MCF7 cell lysate
      Lane 2 : SCARB2 knockout MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 54 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      False colour image of Western blot: Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal staining at 1/20000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab196651 was shown to bind specifically to Scavenging Receptor SRB2. A band was observed at 80 kDa in wild-type MCF7 cell lysates with no signal observed at this size in SCARB2 knockout cell line ab274952 (knockout cell lysate ab275010). To generate this image, wild-type and SCARB2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

    • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      All lanes : Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution

      Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 54 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      The observed MW is consistent with what has been described in the literature (PMID: 21389126, PMID: 22272359 and PMID: 23635510).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Scavenging Receptor SRB2 with ab196651 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on human liver tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    • Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      Western blot - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651) at 1/20000 dilution + Human fetal kidney lysate at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 54 kDa
      Observed band size: 80 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      The observed MW is consistent with what has been described in the literature (PMID: 21389126, PMID: 22272359 and PMID: 23635510).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

      Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Scavenging Receptor SRB2 with ab196651 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    • Flow Cytometry (Intracellular) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      Flow Cytometry (Intracellular) - Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling Scavenging Receptor SRB2 with ab196651 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. 

       

       

    • Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)
      Anti-Scavenging Receptor SRB2 antibody [EPR12081] - C-terminal (ab196651)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab196651? Please let us know so that we can cite the reference in this datasheet.

    ab196651 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab196651.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.