Anti-SCC112 antibody (ab122352)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SCC112 antibody
See all SCC112 primary antibodies -
Description
Rabbit polyclonal to SCC112 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
antigen sequence: GRRPKSESQGNATKNDDLNKPINKGRKRAAVGQESPGGLEAGNAKAPKLQ DLAKKAAPAE RQIDLQ, corresponding to C terminal amino acids 1271-1336 of Human SCC112 (Isoform 1).
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Positive control
- IHC-P: Human colon tissue WB: RT4 and U-251 MG cell lysates; Human plasma and tonsil lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab122352 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250 - 1/500. | |
| IHC-P | 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
Target
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Function
Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. -
Tissue specificity
Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney. -
Sequence similarities
Belongs to the PDS5 family.
Contains 1 HEAT repeat. -
Developmental stage
Cell cycle-regulated with highest level in G2 phase. -
Cellular localization
Nucleus. Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase. - Information by UniProt
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Database links
- Entrez Gene: 23244 Human
- Entrez Gene: 305343 Rat
- Omim: 613200 Human
- SwissProt: Q29RF7 Human
- SwissProt: Q6A026 Mouse
- SwissProt: A4L9P7 Rat
- Unigene: 331431 Human
- Unigene: 278974 Mouse
see all -
Alternative names
- Cell proliferation inducing protein 54 antibody
- Cell proliferation-inducing gene 54 protein antibody
- KIAA0648 antibody
see all
Images
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Western blot - Anti-SCC112 antibody (ab122352)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Western blot - Anti-SCC112 antibody (ab122352)All lanes : Anti-SCC112 antibody (ab122352) at 1/250 dilution
Lane 1 : RT-4 cell line lysate
Lane 2 : U-251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)ab122352, at 1/500, staining SCC112 in paraffin embedded Human colon tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-SCC112 antibody (ab122352)ab122352, at 4 µg/ml, staining SCC112 in U-251 MG cells by Immunofluorescence.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). Read more (PubMed: 30554942) »
