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    scc112-antibody-ab122352.pdf

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Cell Biology Cell Cycle Cell differentiation
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Anti-SCC112 antibody (ab122352)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
  • Immunocytochemistry - Anti-SCC112 antibody (ab122352)
  • Western blot - Anti-SCC112 antibody (ab122352)
  • Western blot - Anti-SCC112 antibody (ab122352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)

Key features and details

  • Rabbit polyclonal to SCC112
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human SCC112 protein (ab161580)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SCC112 antibody
    See all SCC112 primary antibodies
  • Description

    Rabbit polyclonal to SCC112
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: GRRPKSESQGNATKNDDLNKPINKGRKRAAVGQESPGGLEAGNAKAPKLQ DLAKKAAPAE RQIDLQ, corresponding to C terminal amino acids 1271-1336 of Human SCC112 (Isoform 1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human colon, kidney, lymph node and cerebral cortex tissues; WB: NIH/3T3, NBT-II, RT4 and U-251 MG cell lysates; ICC: U-251 MG cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SCC112 protein (ab161580)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab122352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use a concentration of 0.25 - 2 µg/ml.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
ICC
Use a concentration of 0.25 - 2 µg/ml.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
  • Tissue specificity

    Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney.
  • Sequence similarities

    Belongs to the PDS5 family.
    Contains 1 HEAT repeat.
  • Developmental stage

    Cell cycle-regulated with highest level in G2 phase.
  • Cellular localization

    Nucleus. Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase.
  • Target information above from: UniProt accession Q29RF7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 23244 Human
    • Entrez Gene: 305343 Rat
    • Omim: 613200 Human
    • SwissProt: Q29RF7 Human
    • SwissProt: Q6A026 Mouse
    • SwissProt: A4L9P7 Rat
    • Unigene: 331431 Human
    • Unigene: 278974 Mouse
    • Unigene: 24482 Rat
    see all
  • Alternative names

    • Cell proliferation inducing protein 54 antibody
    • Cell proliferation-inducing gene 54 protein antibody
    • KIAA0648 antibody
    • PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) antibody
    • PDS5A antibody
    • PDS5A_HUMAN antibody
    • PIG54 antibody
    • SCC-112 antibody
    • SCC112 antibody
    • Sister chromatid cohesion protein 112 antibody
    • Sister chromatid cohesion protein PDS5 homolog A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling SCC112 with ab122352 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-SCC112 antibody (ab122352)
    Immunocytochemistry - Anti-SCC112 antibody (ab122352)

    Immunocytochemistry analysis of U-251 MG (formally U-373 MG) (human brain glioma cell line) cells labelling SCC112 with ab122352 at 2 µg/mL.

  • Western blot - Anti-SCC112 antibody (ab122352)
    Western blot - Anti-SCC112 antibody (ab122352)
    Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • Western blot - Anti-SCC112 antibody (ab122352)
    Western blot - Anti-SCC112 antibody (ab122352)
    All lanes : Anti-SCC112 antibody (ab122352) at 0.4 µg/ml

    Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) cell lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue labelling SCC112 with ab122352 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling SCC112 with ab122352 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SCC112 with ab122352 at 1/500 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab122352? Please let us know so that we can cite the reference in this datasheet.

ab122352 has been referenced in 1 publication.

  • Meisenberg C  et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942

Customer reviews and Q&As

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