Overview

  • Product name
    Anti-SCC112 antibody
    See all SCC112 primary antibodies
  • Description
    Rabbit polyclonal to SCC112
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    antigen sequence: GRRPKSESQGNATKNDDLNKPINKGRKRAAVGQESPGGLEAGNAKAPKLQ DLAKKAAPAE RQIDLQ, corresponding to C terminal amino acids 1271-1336 of Human SCC112 (Isoform 1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • IHC-P: Human colon tissue WB: RT4 and U-251 MG cell lysates; Human plasma and tonsil lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab122352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • Function
    Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
  • Tissue specificity
    Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney.
  • Sequence similarities
    Belongs to the PDS5 family.
    Contains 1 HEAT repeat.
  • Developmental stage
    Cell cycle-regulated with highest level in G2 phase.
  • Cellular localization
    Nucleus. Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • Cell proliferation inducing protein 54 antibody
    • Cell proliferation-inducing gene 54 protein antibody
    • KIAA0648 antibody
    • PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) antibody
    • PDS5A antibody
    • PDS5A_HUMAN antibody
    • PIG54 antibody
    • SCC-112 antibody
    • SCC112 antibody
    • Sister chromatid cohesion protein 112 antibody
    • Sister chromatid cohesion protein PDS5 homolog A antibody
    see all

Images

  • Western blot - Anti-SCC112 antibody (ab122352)
    Western blot - Anti-SCC112 antibody (ab122352)
    Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • Western blot - Anti-SCC112 antibody (ab122352)
    Western blot - Anti-SCC112 antibody (ab122352)
    All lanes : Anti-SCC112 antibody (ab122352) at 1/250 dilution

    Lane 1 : RT-4 cell line lysate
    Lane 2 : U-251 MG cell line lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCC112 antibody (ab122352)
    ab122352, at 1/500, staining SCC112 in paraffin embedded Human colon tissue by Immunohistochemistry.
  • Immunocytochemistry/ Immunofluorescence - Anti-SCC112 antibody (ab122352)
    Immunocytochemistry/ Immunofluorescence - Anti-SCC112 antibody (ab122352)
    ab122352, at 4 µg/ml, staining SCC112 in U-251 MG cells by Immunofluorescence.

References

ab122352 has not yet been referenced specifically in any publications.

Publishing research using ab122352? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

There are currently no Customer reviews or Questions for ab122352.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up