Key features and details
- Rabbit polyclonal to SCC112
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab17961 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 150 kDa).|
|IP||Use a concentration of 1 - 4 µg/ml.|
FunctionProbable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
Tissue specificityHighest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney.
Sequence similaritiesBelongs to the PDS5 family.
Contains 1 HEAT repeat.
Developmental stageCell cycle-regulated with highest level in G2 phase.
Cellular localizationNucleus. Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase.
- Information by UniProt
- Cell proliferation inducing protein 54 antibody
- Cell proliferation-inducing gene 54 protein antibody
- KIAA0648 antibody
Detection of Human SCC-112 by Western Blot and Immunoprecipitation.
Lane 1: WB; 5
g of NE µ
Lane 2: WB; 20
g of NE µ
Lane 3: IP (with a different antibody) followed by WB; 1 mg of NE
Lane 4: IP (with ab17961) followed by WB; 1 mg of NE
5 to 20
g of nuclear extract (NE) from HeLa cell lysate were used for WB. 1 mg of nuclear extract (NE) from HeLa cell lysate were used for IP. µ
Detection: Chemiluminescence with an exposure time of less than 5 minutes.
Ab17961 was used at a dilution of 1/10,000 for the western blot and 2Detection of Human SCC-112 by Western Blot
µg/mg of extract for immunoprecipitation.
ab17961 has not yet been referenced specifically in any publications.